Evidence map›Paper›PMID 38753197›Full record

ArticleArchives of virology2024

A multiplex digital PCR assay for detection and quantitation of porcine circovirus type 2 and type 3.

Jiangbing Shuai, Kexin Chen, Zhongcai Wang, Ruoxue Zeng, Biao Ma, Mingzhou Zhang, Houhui Song, Xiaofeng Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of virology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jiangbing ShuaiZhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, 310016, China.
Kexin ChenCollege of Animal Science and Technology, Zhejiang Agriculture & Forestry University, Hangzhou, 311300, China.
Zhongcai WangZhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, 310016, China.
Ruoxue ZengZhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, 310016, China.
Biao MaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou, 310018, China.
Mingzhou ZhangZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou, 310018, China.
Houhui SongCollege of Animal Science and Technology, Zhejiang Agriculture & Forestry University, Hangzhou, 311300, China.
Xiaofeng ZhangZhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, 310016, China. zxf@zaiq.org.cn.ORCID http://orcid.org/0009-0002-8005-7092

Funding

the Key Research and Development Program of Zhejiang Province 2021C02060the National Key Research and Development Program of China 2021YFF0602800the research program of General Administration of Customs of China 2021HK59
6 · The paper itself

Abstract

Porcine circovirus (PCV) has become a major pathogen, causing major economic losses in the global pig industry, and PCV type 2 (PCV2) and 3 (PCV3) are distributed worldwide. We designed specific primer and probe sequences targeting PCV2 Cap and PCV3 Rap and developed a multiplex crystal digital PCR (cdPCR) method after optimizing the primer concentration, probe concentration, and annealing temperature. The multiplex cdPCR assay permits precise and differential detection of PCV2 and PCV3, with a limit of detection of 1.39 × 10

Indexed as

Circoviridae InfectionsCircovirusMultiplex Polymerase Chain ReactionSwine DiseasesAnimalsDNA PrimersDNA, ViralReproducibility of ResultsSensitivity and SpecificitySwineDNA PrimersDNA, Viral

Identifiers

PMID38753197

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.