Evidence map›Paper›PMID 38753150›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Microfluidic 3D Cytotoxic Assay.

Hyeri Choi, Sunghun Cheong, Ailian Jin, Dohyun Park, Noo Li Jeon

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Hyeri Choi *Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, South Korea.
Sunghun Cheong *Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, South Korea.
Ailian Jin *Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, South Korea.
Dohyun ParkDepartment of Mechanical Engineering, Seoul National University, Seoul, South Korea.
Noo Li JeonInterdisciplinary Program in Bioengineering, Seoul National University, Seoul, South Korea. njeon@snu.ac.kr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microfluidic-based cytotoxic assays provide high physiological relevance with the potential to replace conventional animal experiments and two-dimensional (2D) assays. Here, a 3D method utilizing a microfluidic platform for analysis of lymphocyte cytotoxicity is introduced in detail, including platform design, cell culture method, real-time cytotoxic assay setup, and image-based analysis. A 2D experimental method is used for comparison, which effectively demonstrates the advantages of 3D microfluidic platforms in closely recapitulating immune responses within the tumor microenvironment. Moreover, a wide range of experimental possibilities and applications using microfluidic 3D cytotoxic assays is introduced in this chapter, along with their capabilities, limitations, and future outlook.

Indexed as

Microfluidic Analytical TechniquesAnimalsCell Culture TechniquesCytotoxicity Tests, ImmunologicHumansLymphocytesMicrofluidicsTumor MicroenvironmentCytotoxic assayImmunotherapyMicrofluidicsOrgan-on-a-chipTumor microenvironment

Identifiers

PMID38753150

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.