Immunochromatography Lateral Flow Strip Enhancement Based on Passive Gold Nanoparticles Conjugation to Detect Schistosma haematobium Antigens in Human Serum.

Mahmoud N El-Shall, Ibrahim Aly, Alaa Samen, Wesam M Salama, Fadi Baakdah

Abstract readEvaluation Study

In one paragraph

Article in Acta parasitologica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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1 citing paper in PubMed.

Development of a monoclonal antibody-based time-resolved fluorescence immunochromatographic assay strip for rapid and quantitative determination of ubiquitin C-terminal hydrolase L1 in serum.Mikrochimica acta · 2026
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5 · Who and what money

Authors and funding

5 authors.

Mahmoud N El-ShallDepartment of Parasitology, Theodor Bilharz Research Institute, Giza, Egypt.

Ibrahim AlyDepartment of Parasitology, Theodor Bilharz Research Institute, Giza, Egypt.

Alaa SamenZoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.

Wesam M SalamaZoology Department, Faculty of Science, Tanta University, Tanta, Egypt. wesam.hassan@science.tanta.edu.eg.ORCID http://orcid.org/0000-0002-3291-5681

Fadi BaakdahDepartment of Medical Laboratory Science, Faculty of Applied Medical Sciences, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.

Funding

STDF 27778

6 · The paper itself

Abstract

purposeThis study aimed to develop and evaluate a lateral flow card for the detection of active Schistosoma haematobium infection.

methodsIn order to prepare the immunochromatography lateral flow strip (ICLFS), antibodies purified from schistosomiasis were conjugated passively with gold nanoparticles using a potassium carbonate buffer.

resultsThe novel ICLFS was able to correctly identify 64 out of 67 samples of schistosomiasis, 6 out of 90 samples of other parasites, and 0 out of 27 control samples. Sensitivity, specificity, negative predictive value (NPV), and positive predictive value (PPV) were 95.5%, 93.3%, 90%, and 91.4% respectively. Comparatively, the sensitivity, specificity, NPV, and PPV of sandwich enzyme-linked immunosorbent assays (ELISA) conjugated with gold nanoparticles (AuNPs) were 91.1%, 88.8%, 85.9%, and 84.4% respectively. The increased sensitivity and specificity of ICLFS produced superior results to those of sandwich ELISA.

conclusionIn conclusion, ICLFS is more beneficial and precise than sandwich ELISA for detection of S. haematobium infection at early stage.

Indexed as

Antigens, HelminthChromatography, AffinityGoldMetal NanoparticlesSchistosoma haematobiumSchistosomiasis haematobiaSensitivity and SpecificityAnimalsAntibodies, HelminthEnzyme-Linked Immunosorbent AssayHumansReagent StripsAntibodies, HelminthAntigens, HelminthGoldReagent StripsAuNPsDiagnosisELISAFlow strip (ICLFS)Immunochromatography lateralSchistosoma haematobium

Identifiers

PMID38753102

DOI10.1007/s11686-024-00841-y

PMCPMC11182813

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