Evidence map›Paper›PMID 38752739›Full record

ArticleJournal of proteome research2024

Offline Two-Dimensional Liquid Chromatography-Mass Spectrometry for Deep Annotation of the Fecal Metabolome Following Fecal Microbiota Transplantation.

Brady G Anderson, Alexander Raskind, Rylan Hissong, Michael K Dougherty, Sarah K McGill, Ajay S Gulati, Casey M Theriot, Robert T Kennedy, Charles R Evans

Abstract read
In one paragraph

Article in Journal of proteome research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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  4. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Brady G AndersonDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.ORCID 0000-0002-1239-3812
Alexander RaskindMichigan Compound Identification Development Core, University of Michigan, Ann Arbor, Michigan 48109, United States.
Rylan HissongMichigan Compound Identification Development Core, University of Michigan, Ann Arbor, Michigan 48109, United States.
Michael K DoughertyDepartment of Medicine, Division of Gastroenterology and Hepatology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.
Sarah K McGillDepartment of Medicine, Division of Gastroenterology and Hepatology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.
Ajay S GulatiDepartment of Medicine, Division of Gastroenterology and Hepatology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.
Casey M TheriotDepartment of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, United States.
Robert T KennedyDepartment of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.ORCID 0000-0003-2447-7471
Charles R EvansMichigan Compound Identification Development Core, University of Michigan, Ann Arbor, Michigan 48109, United States.ORCID 0000-0001-6698-5330

Funding

TR&D 2 Metabolic Labels for Ultraplexed Protein Quantification p. 453P41GM108538 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COON, JOSHUA J · 2016 to 2025
$13.1M
Michigan Compound Identification Development Cores (MCIDC)U2CES030164 · NIEHS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NESVIZHSKII, ALEXEY I · 2018 to 2021
$4.1M
Mechanisms of Gut Microbiota-Driven Paneth Cell RegulationR01DK122042 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI GULATI, AJAY S · 2020 to 2024
$2.4M
NIDDK NIH HHS R01 DK122042NIEHS NIH HHS U2C ES030164NIGMS NIH HHS P41 GM108538
6 · The paper itself

Abstract

Biological interpretation of untargeted LC-MS-based metabolomics data depends on accurate compound identification, but current techniques fall short of identifying most features that can be detected. The human fecal metabolome is complex, variable, incompletely annotated, and serves as an ideal matrix to evaluate novel compound identification methods. We devised an experimental strategy for compound annotation using multidimensional chromatography and semiautomated feature alignment and applied these methods to study the fecal metabolome in the context of fecal microbiota transplantation (FMT) for recurrent

Indexed as

Fecal Microbiota TransplantationFecesMetabolomeMetabolomicsTandem Mass SpectrometryBile Acids and SaltsChromatography, LiquidClostridioides difficileClostridium InfectionsHumansLiquid Chromatography-Mass SpectrometryBile Acids and Saltsbile acidsC. diffClostridioides difficilecompound identificationHILICLC × LCLC-MSMS/MSRPLCuntargeted metabolomics

Identifiers

PMID38752739
PMCPMC12177891

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.