Evidence map›Paper›PMID 38749444›Full record

ArticleCell reports methods2024

Methods for making and observing model lipid droplets.

Sonali A Gandhi, Shahnaz Parveen, Munirah Alduhailan, Ramesh Tripathi, Nasser Junedi, Mohammad Saqallah, Matthew A Sanders, Peter M Hoffmann, Katherine Truex, James G Granneman and 1 more

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In one paragraph

Article in Cell reports methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Sonali A GandhiDepartment of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA.
Shahnaz ParveenDepartment of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA.
Munirah AlduhailanDepartment of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA.
Ramesh TripathiDepartment of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA.
Nasser JunediDepartment of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA.
Mohammad SaqallahDepartment of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA.
Matthew A SandersCenter for Molecular Medicine and Genetics, School of Medicine, Wayne State University, Detroit, MI 40201, USA; Center for Integrative Metabolic and Endocrine Research, School of Medicine, Wayne State University, Detroit, MI 48201, USA.
Peter M HoffmannDepartment of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA; Physical Sciences Department, Embry-Riddle Aeronautical University, Daytona Beach, FL 32114, USA.
Katherine TruexDepartment of Physics, United States Naval Academy, Annapolis, MD 21402, USA.
James G GrannemanCenter for Molecular Medicine and Genetics, School of Medicine, Wayne State University, Detroit, MI 40201, USA; Center for Integrative Metabolic and Endocrine Research, School of Medicine, Wayne State University, Detroit, MI 48201, USA.
Christopher V KellyDepartment of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA; Center for Integrative Metabolic and Endocrine Research, School of Medicine, Wayne State University, Detroit, MI 48201, USA. Electronic address: cvkelly@wayne.edu.

Funding

Analysis of Lipolytic Trafficking in Adipocytes.R01DK076629 · NIDDK · WAYNE STATE UNIVERSITY · PI James G Granneman, Christopher V Kelly · 2009 to 2026
$7.1M
NIDDK NIH HHS R01 DK076629
6 · The paper itself

Abstract

We present methods for making and testing the membrane biophysics of model lipid droplets (LDs). Methods are described for imaging LDs ranging in size from 0.1 to 40 μm in diameter with high-resolution microscopy and spectroscopy. With known LD compositions, membrane binding, sorting, diffusion, and tension were measured via fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching (FRAP), fluorescence lifetime imaging microscopy (FLIM), atomic force microscopy (AFM), and imaging flow cytometry. Additionally, a custom, small-volume pendant droplet tensiometer is described and used to measure the association of phospholipids to the LD surface. These complementary, cross-validating methods of measuring LD membrane behavior reveal the interplay of biophysical processes on lipid droplet monolayers.

Indexed as

Lipid DropletsFlow CytometryFluorescence Recovery After PhotobleachingHumansMicroscopy, Atomic ForceMicroscopy, FluorescenceSpectrometry, FluorescenceAFMatomic force microscopyCP: BiotechnologyCP: Cell biologydiffusionFCSFLIMfluorescence correlation spectroscopyfluorescence lifetime imaging microscopyfluorescence microscopyfluorescence recovery after photobleachingFRAPmodel lipid dropletspendant droplet tensiometryphospholipid monolayerssortingtension

Identifiers

PMID38749444
PMCPMC11133809

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.