Evidence map›Paper›PMID 38748684›Full record

ArticleJournal of leukocyte biology2024

Treponema pallidum recombinant protein Tp0768 enhances the ability of HUVECs to promote neutrophil chemotaxis through the TLR2/ER stress signaling pathway.

Ting Cao, Yue Li, Xiangping Zhou, Yun Tang, Bisha He, Qian Cao, Yibao Hu, En Chen, Yumeng Li, Xiaoping Xie and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of leukocyte biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Putative mechanisms of ocular inflammation in syphilis.Journal of ophthalmic inflammation and infection · 2026
    Review
  2. Article
  3. Frontiers in immunology · 2026
    Review
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Ting CaoDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.ORCID 0000-0002-5436-6724
Yue LiDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.
Xiangping ZhouDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.
Yun TangDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.ORCID 0000-0003-0211-7360
Bisha HeDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.
Qian CaoDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.
Yibao HuDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.
En ChenDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.
Yumeng LiDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.
Xiaoping XieDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.ORCID 0009-0008-3196-7843
Feijun ZhaoInstitute of Pathogenic Biology and Key Laboratory of Special Pathogen Prevention and Control of Hunan Province, Hengyang Medical College, University of South China, No.28, Changsheng West Road, Hengyang 421001, China.
Xiaopeng LanDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.
Shuangquan LiuDepartment of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, Hunan Province Clinical Research Center for Accurate Diagnosis and Treatment of High-Incidence Sexually Transmitted Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, No.69, Chuanshang Road, Hengyang 421001, Hunan, China.ORCID 0000-0002-2690-2665

Funding

Natural Science Foundation of Hunan Province 2022JJ30532NIH HHSUniversity of South China Innovation Foundation for Postgraduate 213YXC016
6 · The paper itself

Abstract

Neutrophils are essential cells involved in inflammation. However, the specific mechanism of neutrophil chemotaxis induced by Treponema pallidum remains unknown. In this study, human umbilical vein endothelial cells (HUVECs) were utilized as target cells to investigate the expression levels of chemokines when stimulated with different concentrations of Tp0768 (also known as TpN44.5 or TmpA, a T. pallidum infection dependent antigen). The results indicated that Tp0768 treatment enhanced neutrophil chemotaxis in HUVECs, which was closely associated with the expression levels of CXCL1, CXCL2, and CXCL8 (also known as interleukin-8). At the same time, the results show that the Toll-like receptor 2 (TLR2) signaling pathway is activated and that endoplasmic reticulum (ER) stress occurs. Furthermore, the findings revealed that the use of protein kinase RNA-like endoplasmic reticulum kinase (PERK) and immunoglobulin-regulated enhancer 1 (IRE1) inhibitors reduced the expression levels of CXCL1, CXCL2, and CXCL8. Additionally, inhibiting TLR2 significantly decreased the expression levels of ER stress-related proteins (PERK and IRE1), CXCL1, CXCL2, and CXCL8. Consequently, neutrophil chemotaxis was significantly inhibited after treatment with TLR2, PERK, and IRE1 inhibitors. These findings shed light on the role of Tp0768 in enhancing neutrophil chemotaxis in endothelial cells, providing a foundation for further exploration of syphilis pathogenesis and offering a new direction for the diagnosis and treatment of T. pallidum infection.

Indexed as

Endoplasmic Reticulum StressHuman Umbilical Vein Endothelial CellsNeutrophilsSignal TransductionToll-Like Receptor 2Treponema pallidumAntigens, BacterialBacterial ProteinsChemotaxisChemotaxis, LeukocyteeIF-2 KinaseHumansInterleukin-8Protein Serine-Threonine KinasesRecombinant ProteinsAntigens, BacterialBacterial ProteinsEIF2AK3 protein, humaneIF-2 KinaseInterleukin-8Protein Serine-Threonine KinasesRecombinant ProteinsTLR2 protein, humanToll-Like Receptor 2chemotaxisER stressinflammationneutrophilsTLR2treponema pallidum

Identifiers

PMID38748684
PMCPMC11531811

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.