ArticleRice (New York, N.Y.)2024
The DEP1 Mutation Improves Stem Lodging Resistance and Biomass Saccharification by Affecting Cell Wall Biosynthesis in Rice.
Article in Rice (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- Advances in Genes Associated with Straw Degradation in Rice.Life (Basel, Switzerland) · 2026Review
- Knockout of RRS1 Enhances Culm Mechanical Strength and Grain Yield in Rice.Plant, cell & environment · 2026Article
- Enhanced saccharification yields from rice straw by senescence-induced expression of a cytokinin biosynthesis gene in intragenic rice plants.Plant biotechnology (Tokyo, Japan) · 2026Article
- Analysis of Genetic Variation of Rice Straw Characteristics and Its Influence on Biomass.Plant direct · 2026Article
- Tissue culture optimization and genome editing for yield improvement of an Indian rice landrace Chittimuthyalu.Transgenic research · 2025Article
- Article
- Genome-Wide Association Study to Identify Soybean Lodging Resistance Loci and Candidate Genes.International journal of molecular sciences · 2025Article
- Precise genome editing of Dense and Erect Panicle 1 promotes rice sheath blight resistance and yield production in japonica rice.Plant biotechnology journal · 2025Article
- Telomere-to-telomere, gap-free genome of mung bean (Horticulture research · 2025Article
- The knockout of Gγ subunit HvGS3 by CRISPR/Cas9 gene editing improves the lodging resistance of barley through dwarfing and stem strengthening.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
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13 authors.
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Abstract
backgroundPlant cell walls have evolved precise plasticity in response to environmental stimuli. The plant heterotrimeric G protein complexes could sense and transmit extracellular signals to intracellular signaling systems, and activate a series of downstream responses. dep1 (Dense and Erect Panicles 1), the gain-of-function mutation of DEP1 encoding a G protein γ subunit, confers rice multiple improved agronomic traits. However, the effects of DEP1 on cell wall biosynthesis and wall-related agronomic traits remain largely unknown.
resultsIn this study, we showed that the DEP1 mutation affects cell wall biosynthesis, leading to improved lodging resistance and biomass saccharification. The DEP1 is ubiquitously expressed with a relatively higher expression level in tissues rich in cell walls. The CRISPR/Cas9 editing mutants of DEP1 (dep1-cs) displayed a significant enhancement in stem mechanical properties relative to the wild-type, leading to a substantial improvement in lodging resistance. Cell wall analyses showed that the DEP1 mutation increased the contents of cellulose, hemicelluloses, and pectin, and reduced lignin content and cellulose crystallinity (CrI). Additionally, the dep1-cs seedlings exhibited higher sensitivity to cellulose biosynthesis inhibitors, 2,6-Dichlorobenzonitrile (DCB) and isoxaben, compared with the wild-type, confirming the role of DEP1 in cellulose deposition. Moreover, the DEP1 mutation-mediated alterations of cell walls lead to increased enzymatic saccharification of biomass after the alkali pretreatment. Furthermore, the comparative transcriptome analysis revealed that the DEP1 mutation substantially altered expression of genes involved in carbohydrate metabolism, and cell wall biosynthesis.
conclusionsOur findings revealed the roles of DEP1 in cell wall biosynthesis, lodging resistance, and biomass saccharification in rice and suggested genetic modification of DEP1 as a potential strategy to develop energy rice varieties with high lodging resistance.
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