Evidence map›Paper›PMID 38748252›Full record

ArticleMicrobial ecology2024

Design and Validation of Primer Sets for the Detection and Quantification of Antibiotic Resistance Genes in Environmental Samples by Quantitative PCR.

Lizandra Perez-Bou, Alejandro Gonzalez-Martinez, Juan J Cabrera, Belen Juarez-Jimenez, Belen Rodelas, Jesus Gonzalez-Lopez, David Correa-Galeote

Abstract readValidation Study
In one paragraph

Article in Microbial ecology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. A Health Threat from Farm to Fork: Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2024
    Article
  5. Tomato SlWRKY3 Negatively RegulatesPlants (Basel, Switzerland) · 2024
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Lizandra Perez-BouEnvironmental Microbiology Group, Department of Microbiology and Virology, Faculty of Biology, University of Havana, Havana, Cuba.
Alejandro Gonzalez-MartinezMicrobiology and Environmental Technologies Section, Water Research Institute, University of Granada, Granada, Spain.
Juan J CabreraNitrogen Metabolism Group, Zaidín Experimental Station, Spanish National Research Council, EEZ-CSIC, Granada, Spain.
Belen Juarez-JimenezMicrobiology and Environmental Technologies Section, Water Research Institute, University of Granada, Granada, Spain.
Belen RodelasMicrobiology and Environmental Technologies Section, Water Research Institute, University of Granada, Granada, Spain.
Jesus Gonzalez-LopezMicrobiology and Environmental Technologies Section, Water Research Institute, University of Granada, Granada, Spain.
David Correa-GaleoteMicrobiology and Environmental Technologies Section, Water Research Institute, University of Granada, Granada, Spain. dcorrea@ugr.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The high prevalence of antibiotic resistant bacteria (ARB) in several environments is a great concern threatening human health. Particularly, wastewater treatment plants (WWTP) become important contributors to the dissemination of ARB to receiving water bodies, due to the inefficient management or treatment of highly antibiotic-concentrated wastewaters. Hence, it is vital to develop molecular tools that allow proper monitoring of the genes encoding resistances to these important therapeutic compounds (antibiotic resistant genes, ARGs). For an accurate quantification of ARGs, there is a need for sensitive and robust qPCR assays supported by a good design of primers and validated protocols. In this study, eleven relevant ARGs were selected as targets, including aadA and aadB (conferring resistance to aminoglycosides); ampC, bla

Indexed as

Anti-Bacterial AgentsDNA PrimersGenes, BacterialReal-Time Polymerase Chain ReactionWastewaterBacteriaDrug Resistance, BacterialAnti-Bacterial AgentsDNA PrimersWastewaterAntibiotic resistance genesAntibioticsAssay optimizationPrimer designqPCRWWTPs

Identifiers

PMID38748252
PMCPMC11096201

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.