Evidence map›Paper›PMID 38747873›Full record

ArticleSao Paulo medical journal = Revista paulista de medicina2024

hTERT gene methylation in circulating DNA, tumor, and surrounding tissue in breast cancer: a prospective study.

Luiz Fernando de Queiroz, Marcelo Soares da Mota E Silva, Heitor Siffert Pereira de Souza, Siane Lopes Bittencourt Rosas, Maria da Glória da Costa Carvalho

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PubMed Full text at PMC Publisher
In one paragraph

Article in Sao Paulo medical journal = Revista paulista de medicina, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Luiz Fernando de QueirozMD, PhD. Molecular Biologist, Department of Pathology, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (RJ), Brazil.ORCID 0000-0002-6139-0597
Marcelo Soares da Mota E SilvaMD, PhD. Molecular Biologist, Department of Pathology, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (RJ), Brazil.ORCID 0000-0003-0981-5233
Heitor Siffert Pereira de SouzaMD, PhD. Physician and Full Professor, Department of Internal Medicine, School of Medicine, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (RJ), Brazil.ORCID 0000-0002-3647-7324
Siane Lopes Bittencourt RosasMD, PhD. Molecular Biologist, Department of Internal Medicine, School of Medicine, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (RJ), Brazil.ORCID 0000-0002-4814-0868
Maria da Glória da Costa CarvalhoMD, PhD. Physician and Associate Professor, Department of Pathology, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (RJ), Brazil.ORCID 0000-0001-5890-8095

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) 303544/2020-1Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) E-26/010/001321/2019
6 · The paper itself

Abstract

backgroundThe human telomerase reverse transcriptase (hTERT) enzyme, encoded by the hTERT gene, synthesizes protective telomeric sequences on chromosomes and plays a fundamental role in cancer formation. Methylation of the hTERT gene has an upregulatory effect, increasing hTERT enzyme synthesis and allowing continuous tumor cell division.

objectiveIn a group of patients with breast cancer, we aimed to analyze the methylation status of hTERT in the tumor, surrounding tissue, and circulating free deoxyribonucleic acid (cfDNA) of blood collected on the day of mastectomy and then approximately one year later. DESIGN AND

settingA prospective study was conducted at a university hospital in Rio de Janeiro, Brazil.

methodsSamples were collected from 15 women with breast cancer on the day of mastectomy and approximately one year postoperatively. cfDNA was analyzed by sodium bisulfite conversion, followed by polymerase chain reaction, electrophoresis, and silver nitrate staining.

resultsMethylation of hTERT was detected in the tumors and surrounding tissues of all 15 patients. Five patients displayed hTERT methylation in the cfDNA from the blood of the first collection. Of the ten patients who returned for the second collection, three showed methylation. Two patients with methylation in the first collection did not display methylation in the second collection. One patient with no methylation in the first collection displayed methylation in the second collection, and one patient had a diminished level of methylation in the second collection.

conclusionOnly one-third of patients displayed methylation in their cfDNA, which may be related to the success of chemotherapy.

Indexed as

Breast NeoplasmsDNA MethylationTelomeraseAdultAgedBiomarkers, TumorFemaleHumansMastectomyMiddle AgedPolymerase Chain ReactionProspective StudiesBiomarkers, TumorTelomeraseTERT protein, human

Identifiers

PMID38747873
PMCPMC11087006

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.