Evidence map›Paper›PMID 38746439›Full record

ArticlebioRxiv : the preprint server for biology2024

A temperature-sensitive and interferon-silent Sendai virus vector for CRISPR-Cas9 delivery and gene editing in primary human cells.

Christian S Stevens, Jillian Carmichael, Ruth Watkinson, Shreyas Kowdle, Rebecca A Reis, Kory Hamane, Jason Jang, Arnold Park, Olivier Pernet, Wannisa Khamaikawin and 5 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Christian S StevensDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.ORCID 0000-0003-2472-6984
Jillian CarmichaelDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.ORCID 0000-0001-5270-5916
Ruth WatkinsonDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.
Shreyas KowdleDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.
Rebecca A ReisDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.
Kory HamaneUCLA School of Nursing, Los Angeles, California, 90095.
Jason JangUCLA School of Nursing, Los Angeles, California, 90095.
Arnold ParkDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.
Olivier PernetUCLA School of Nursing, Los Angeles, California, 90095.
Wannisa KhamaikawinUCLA School of Nursing, Los Angeles, California, 90095.
Patrick HongDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.
Patricia ThibaultDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.ORCID 0000-0002-7313-0848
Aditya GowlikarDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.
Dong Sung AnUCLA School of Nursing, Los Angeles, California, 90095.
Benhur LeeDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029.ORCID 0000-0003-0760-1709

Funding

TRAINING PROGRAM: MECHANISMS OF VIRUS-HOST INTERACTIONST32AI007647 · NIAID · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI Domenico Tortorella · 2000 to 2026
$11.5M
Mount Sinai Medical Scientist Training ProgramT32GM146636 · NIGMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Talia H Swartz · 2022 to 2026
$6.8M
Efficient Sendai virus mediated CRISPR/Cas9 gene editing to protect hematopoietic stem cells from HIVR01AI138921 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AN, DONG SUNG, LEE, BENHUR · 2018 to 2022
$3.1M
Tropism, pathogenicity, and potential for zoonotic spillover of emergent henipa- and henipa-like virusesR01AI123449 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI LEE, BENHUR · 2016 to 2020
$2.7M
Human Parainfluenza Virus 3 Infection in a Novel Human Lung Tissue ModelR21AI149033 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI LEE, BENHUR, LIM, JEAN KYOU · 2020 to 2021
$463k
Metabolic and Immune Consequences of Antibiotic-Related Microbiome Alterations during TB TreatmentR21AI148033 · NIAID · NORTHWESTERN UNIVERSITY · PI MAIGA, MAMOUDOU · 2019 to 2020
$440k
NIAID NIH HHS R01 AI123449NIAID NIH HHS R01 AI138921NIAID NIH HHS R21 AI148033NIAID NIH HHS R21 AI149033NIAID NIH HHS T32 AI007647NIGMS NIH HHS T32 GM146636
6 · The paper itself

Abstract

The transformative potential of gene editing technologies hinges on the development of safe and effective delivery methods. In this study, we developed a temperature-sensitive and interferon-silent Sendai virus (ts SeV) as a novel delivery vector for CRISPR-Cas9 and for efficient gene editing in sensitive human cell types without inducing IFN responses. ts SeV demonstrates unprecedented transduction efficiency in human CD34+ hematopoietic stem and progenitor cells (HSPCs) including transduction of the CD34+/CD38-/CD45RA-/CD90+(Thy1+)/CD49f

Indexed as

CCR5CRISPR/Cas9Gene editinghematopoietic stem and progenitor cellsHIVParamyxoviridaeSendai virusviral vector

Identifiers

PMID38746439
PMCPMC11092779

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.