ArticleZeitschrift fur Parasitenkunde (Berlin, Germany)1985
Ultrastructural changes of Trichomonas vaginalis prior and after cryopreservation.
Article in Zeitschrift fur Parasitenkunde (Berlin, Germany), 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed, 3 citations in OpenAlex.
- Effects of dimethylsulfoxide and the deep-freezing process on the infectivity, motility, and ultrastructure of Trypanosoma cruzi.Parasitology research · 1988Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fine structural changes of Trichomonas vaginalis are described prior and after the freezing process in liquid nitrogen. Dimethyl sulfoxide (DMSO) used as the cryoprotectant caused distinct alterations of the cytoplasm when trichomonads were equilibrated with 5% DMSO under various experimental conditions. Changes were bubble-like protrusions, fissuration and/or vacuolation of the cytoplasm, doubling and removal or/and rupture of the cell membrane. Apart from these findings cryopreservation caused marked alterations on the hydrogenosomes, such as condensation and flocculence of the usually homogeneous contents; in addition numerous hydrogenosomes fused while loosing membrane at the site of fusion. However, several parasites revealed normal hydrogenosomes after the freezing process. It is assumed that these organisms survived freezing and thawing as demonstrated by successful cultivation of recovered trichomonads.
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