Evidence map›Paper›PMID 38744971›Full record

ArticleEMBO reports2024

The kinase ZYG-1 phosphorylates the cartwheel protein SAS-5 to drive centriole assembly in C. elegans.

Prabhu Sankaralingam, Shaohe Wang, Yan Liu, Karen F Oegema, Kevin F O'Connell

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EMBO reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. The embryonic lethal mutationmicroPublication biology · 2025
    Article
  4. ThebioRxiv : the preprint server for biology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Prabhu SankaralingamLaboratory of Biochemistry and Genetics, National Institutes of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA. prabhu.sankaralingam@nih.gov.ORCID 0000-0001-9801-0845
Shaohe WangJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID 0000-0001-9522-2359
Yan LiuLaboratory of Biochemistry and Genetics, National Institutes of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA.
Karen F OegemaDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California, San Diego, La Jolla, CA, 92093, USA.
Kevin F O'ConnellLaboratory of Biochemistry and Genetics, National Institutes of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA. kevino@nih.gov.ORCID 0000-0002-9789-288X

Funding

Centrosome Maturation and Duplication in the C. elegans EmbryoZIADK024151 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI O'CONNELL, KEVIN · 2009 to 2025
$10.6M
Analysis of Centrosome DynamicsR01GM074207 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OEGEMA, KAREN F · 2006 to 2025
$6.6M
Centrosome Maturation /Duplication in C elegans EmbryoZ01DK024151 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI O'CONNELL, KEVIN F · 2003 to 2008
$526k
HHS | National Institutes of Health (NIH) GM074207HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) DK024151-10Intramural NIH HHS Z01 DK024151NIGMS NIH HHS R01 GM074207
6 · The paper itself

Abstract

Centrioles organize centrosomes, the cell's primary microtubule-organizing centers (MTOCs). Centrioles double in number each cell cycle, and mis-regulation of this process is linked to diseases such as cancer and microcephaly. In C. elegans, centriole assembly is controlled by the Plk4 related-kinase ZYG-1, which recruits the SAS-5-SAS-6 complex. While the kinase activity of ZYG-1 is required for centriole assembly, how it functions has not been established. Here we report that ZYG-1 physically interacts with and phosphorylates SAS-5 on 17 conserved serine and threonine residues in vitro. Mutational scanning reveals that serine 10 and serines 331/338/340 are indispensable for proper centriole assembly. Embryos expressing SAS-5

Indexed as

Caenorhabditis elegansCaenorhabditis elegans ProteinsCell Cycle ProteinsCentriolesAmino Acid SequenceAnimalsPhosphorylationProtein BindingProtein KinasesProtein Serine-Threonine KinasesSerineCaenorhabditis elegans ProteinsCell Cycle ProteinsProtein KinasesProtein Serine-Threonine KinasesSAS-5 protein, C elegansSAS-6 protein, C elegansSerinezyg-1 protein, C elegansC. elegansCentrioleKinaseProtein–Protein Interaction

Identifiers

PMID38744971
PMCPMC11169420

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.