Evidence map›Paper›PMID 38744831›Full record

ArticleNature communications2024

Long-read powered viral metagenomics in the oligotrophic Sargasso Sea.

Joanna Warwick-Dugdale, Funing Tian, Michelle L Michelsen, Dylan R Cronin, Karen Moore, Audrey Farbos, Lauren Chittick, Ashley Bell, Ahmed A Zayed, Holger H Buchholz and 5 more

Abstract read
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
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  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Joanna Warwick-Dugdale *School of Biosciences, University of Exeter, Exeter, Devon, EX4 4SB, UK. jo.warwick@gmail.com.ORCID http://orcid.org/0000-0001-5242-6706
Funing Tian *Center of Microbiome Science and Department of Microbiology, Ohio State University, Columbus, OH, 43210, USA.ORCID http://orcid.org/0000-0002-7217-3125
Michelle L MichelsenSchool of Biosciences, University of Exeter, Exeter, Devon, EX4 4SB, UK.
Dylan R CroninCenter of Microbiome Science and Department of Microbiology, Ohio State University, Columbus, OH, 43210, USA.
Karen MooreSchool of Biosciences, University of Exeter, Exeter, Devon, EX4 4SB, UK.ORCID http://orcid.org/0000-0002-0146-0653
Audrey FarbosSchool of Biosciences, University of Exeter, Exeter, Devon, EX4 4SB, UK.
Lauren ChittickCenter of Microbiome Science and Department of Microbiology, Ohio State University, Columbus, OH, 43210, USA.
Ashley BellSchool of Biosciences, University of Exeter, Exeter, Devon, EX4 4SB, UK.ORCID http://orcid.org/0000-0003-0198-2205
Ahmed A ZayedCenter of Microbiome Science and Department of Microbiology, Ohio State University, Columbus, OH, 43210, USA.ORCID http://orcid.org/0000-0003-2793-2679
Holger H BuchholzSchool of Biosciences, University of Exeter, Exeter, Devon, EX4 4SB, UK.
Luis M BolanosSchool of Biosciences, University of Exeter, Exeter, Devon, EX4 4SB, UK.ORCID http://orcid.org/0000-0002-0832-016X
Rachel J ParsonsBermuda Institute of Ocean Sciences, St.George's, GE, 01, Bermuda.
Michael J AllenSchool of Biosciences, University of Exeter, Exeter, Devon, EX4 4SB, UK.ORCID http://orcid.org/0000-0001-8504-7171
Matthew B SullivanCenter of Microbiome Science and Department of Microbiology, Ohio State University, Columbus, OH, 43210, USA.ORCID http://orcid.org/0000-0001-8398-8234
Ben TempertonSchool of Biosciences, University of Exeter, Exeter, Devon, EX4 4SB, UK. b.temperton@exeter.ac.uk.ORCID http://orcid.org/0000-0002-3667-8302

Funding

Gordon and Betty Moore Foundation (Gordon E. and Betty I. Moore Foundation) 3790Gordon and Betty Moore Foundation (Gordon E. and Betty I. Moore Foundation) 5488National Science Foundation (NSF) ABI#1759874National Science Foundation (NSF) OCE#1829640National Science Foundation (NSF) OCE#1829831National Science Foundation (NSF) OCE#2019589RCUK | Natural Environment Research Council (NERC) NE/L002434/1RCUK | Natural Environment Research Council (NERC) NE/P008534/1RCUK | Natural Environment Research Council (NERC) NE/R010935/1Wellcome Trust
6 · The paper itself

Abstract

Dominant microorganisms of the Sargasso Sea are key drivers of the global carbon cycle. However, associated viruses that shape microbial community structure and function are not well characterised. Here, we combined short and long read sequencing to survey Sargasso Sea phage communities in virus- and cellular fractions at viral maximum (80 m) and mesopelagic (200 m) depths. We identified 2,301 Sargasso Sea phage populations from 186 genera. Over half of the phage populations identified here lacked representation in global ocean viral metagenomes, whilst 177 of the 186 identified genera lacked representation in genomic databases of phage isolates. Viral fraction and cell-associated viral communities were decoupled, indicating viral turnover occurred across periods longer than the sampling period of three days. Inclusion of long-read data was critical for capturing the breadth of viral diversity. Phage isolates that infect the dominant bacterial taxa Prochlorococcus and Pelagibacter, usually regarded as cosmopolitan and abundant, were poorly represented.

Indexed as

BacteriophagesMetagenomeMetagenomicsOceans and SeasSeawaterBacteriaGenome, ViralMicrobiotaPhylogenyProchlorococcus

Identifiers

PMID38744831
PMCPMC11094077

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.