Evidence map›Paper›PMID 38744718›Full record

ArticleArchives of microbiology2024

Exploration of genes associated with induction of the viable but non-culturable state of Campylobacter jejuni.

Yurina Ohno, Md Matiur Rahman, Hiroe Maruyama, Yasuo Inoshima, Ayaka Okada

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yurina OhnoLaboratory of Food and Environmental Hygiene, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Md Matiur RahmanLaboratory of Food and Environmental Hygiene, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Hiroe MaruyamaNagaragawa Research Center, API Co., Ltd, Gifu, Japan.
Yasuo InoshimaLaboratory of Food and Environmental Hygiene, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Ayaka OkadaLaboratory of Food and Environmental Hygiene, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan. okada.ayaka.i2@f.gifu-u.ac.jp.

Funding

Japan Society for the Promotion of Science 23K14079
6 · The paper itself

Abstract

Campylobacter jejuni is known to enter a viable but non-culturable (VBNC) state when exposed to environmental stresses. Microarray and quantitative real-time polymerase chain reaction (qPCR) analyses were performed to elucidate the genes related to the induction of the VBNC state. The C. jejuni NCTC11168 strain was cultured under low-temperature or high-osmotic stress conditions to induce the VBNC state. mRNA expression in the VBNC state was investigated using microarray analysis, and the gene encoding peptidoglycan-associated lipoprotein, Pal, was selected as the internal control gene using qPCR analysis and software. The three genes showing particularly large increases in mRNA expression, cj1500, cj1254, and cj1040, were involved in respiration, DNA repair, and transporters, respectively. However, formate dehydrogenase encoded by cj1500 showed decreased activity in the VBNC state. Taken together, C. jejuni actively changed its mRNA expression during induction of the VBNC state, and protein activities did not always match the mRNA expression levels.

Indexed as

Bacterial ProteinsCampylobacter jejuniGene Expression Regulation, BacterialGene Expression ProfilingMicrobial ViabilityOsmotic PressureReal-Time Polymerase Chain ReactionRNA, MessengerStress, PhysiologicalBacterial ProteinsRNA, MessengerCampylobacter jejuniInternal control geneMicroarrayViable but non-culturable state

Identifiers

PMID38744718
PMCPMC11093796

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.