Evidence map›Paper›PMID 38744310›Full record

ArticleReproduction (Cambridge, England)2024

Highly expressed lncRNA H19 in endometriosis promotes aerobic glycolysis and histone lactylation.

Xiaoyang Wen, Jingyang Zhang, Zihan Xu, Muzi Li, Xiaotong Dong, Yanbo Du, Zhen Xu, Lei Yan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproduction (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiaoyang WenInstitute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China.
Jingyang ZhangInstitute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China.
Zihan XuInstitute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China.
Muzi LiInstitute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China.
Xiaotong DongInstitute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China.
Yanbo DuInstitute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China.
Zhen XuCenter for Medical Genetics and Prenatal Diagnosis, Shandong Provincial Maternal and Child Health Care Hospital, Jinan, Shandong, China.
Lei YanInstitute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, China.ORCID 0000-0001-6548-1317

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In brief: Abnormal glucose metabolism may be involved in the pathogenesis of endometriosis. The present study identifies that highly expressed H19 leads to increased aerobic glycolysis and histone lactylation levels in endometriosis. Abstract: Previous studies from our group and others have shown increased IncRNA H19 expression in both the eutopic endometrium and the ectopic endometriosis tissue during endometriosis. In this study, we use immunofluorescence, immunohistochemistry, and protein quantification to determine that levels of aerobic glycolysis and histone lactylation are increased in endometriosis tissues. In human endometrial stromal cells, we found that high H19 expression resulted in abnormal glucose metabolism by examining the levels of glucose, lactate, and ATP and measuring protein levels of enzymes that participate in glycolysis. At the same time, immunofluorescence and western blotting demonstrated increased histone lactylation in H19 overexpressing cells. Altering aerobic glycolysis and histone lactylation levels through the addition of sodium lactate and 2-deoxy-d-glucose demonstrated that increased aerobic glycolysis and histone lactylation levels resulted in enhanced cell proliferation and cell migration, contributing to endometriosis. To validate these findings in vivo, we constructed an endometriosis mouse model, demonstrating similar changes in endometriosis tissues in vivo. Both aerobic glycolysis and histone lactylation levels were elevated in endometriotic lesions. Taken together, these data demonstrate elevated expression levels of H19 in endometriosis patients promote abnormal glucose metabolism and elevated histone lactylation levels in vivo, enhancing cell proliferation and migration and promoting the progression of endometriosis. Our study provides a functional link between H19 expression and histone lactylation and glucose metabolism in endometriosis, providing new insights into disease mechanisms that could result in novel therapeutic approaches.

Indexed as

EndometriosisGlycolysisHistonesRNA, Long NoncodingAdultAnimalsCell ProliferationEndometriumFemaleGlucoseHumansMiceGlucoseH19 long non-coding RNAHistonesRNA, Long Noncoding

Identifiers

PMID38744310
PMCPMC11301422

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.