Evidence map›Paper›PMID 38743731›Full record

ArticlePloS one2024

In vitro and in vivo burn healing study of standardized propolis: Unveiling its antibacterial, antioxidant and anti-inflammatory actions in relation to its phytochemical profiling.

Dina M El-Kersh, Rania F Abou El-Ezz, Eman Ramadan, Reham F El-Kased

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Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Dina M El-KershFaculty of Pharmacy, Pharmacognosy Department, The British University in Egypt, Cairo, Egypt.
Rania F Abou El-EzzFaculty of Pharmacy, Pharmacognosy Department, Misr International University, Cairo, Egypt.
Eman RamadanFaculty of Pharmacy, Department of Pharmacology and Toxicology, The British University in Egypt, Cairo, Egypt.
Reham F El-KasedCenter for Drug Research and Development (CDRD), The British University in Egypt, Cairo, Egypt.ORCID 0000-0001-6964-2560

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNatural propolis has been used since decades owing to its broad-spectrum activities. Burn injuries are a global health problem with negative impacts on communities. Bacterial infections usually accompany burns, which demand implementation of antibiotics. Antibiotics abuse led to emergence of microbial drug resistance resulting in poor treatment outcomes. In such instances, the promising alternative would be natural antimicrobials such as propolis.

objectiveFull chemical profiling of propolis and evaluation of in vitro antibacterial, antioxidant and anti-inflammatory activities as well as in vivo burn healing properties.

methodsChemical profiling of propolis was performed using Liquid chromatography (UHPLC/MS-PDA and HPLC-PDA). In vitro assessment was done using Disc Diffusion susceptibility test against Staphylococcus aureus and infected burn wound mice model was used for in vivo assessment. In vitro antioxidant properties of propolis were assessed using DPPH, ABTS and FRAP techniques. The anti-inflammatory effect of propolis was assessed against lipopolysaccharide/interferon-gamma mediated inflammation.

resultsUHPLC/MS-PDA results revealed identification of 71 phytochemicals, mainly flavonoids. Upon flavonoids quantification (HPLC-PDA), Pinocembrin, chrysin and galangin recorded high content 21.58±0.84, 22.73±0.68 and 14.26±0.70 mg/g hydroalcoholic propolis extract, respectively. Propolis showed concentration dependent antibacterial activity in vitro and in vivo burn healing via wound diameter reduction and histopathological analysis without signs of skin irritation in rabbits nor sensitization in guinea pigs. Propolis showed promising antioxidant IC50 values 46.52±1.25 and 11.74±0.26 μg/mL whereas FRAP result was 445.29±29.9 μM TE/mg. Anti-inflammatory experiment results showed significant increase of Toll-like receptor 4 (TLR4), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) mRNA levels. Nitric oxide and iNOS were markedly increased in Griess assay and western blot respectively. However, upon testing propolis against LPS/IFN-γ-mediated inflammation, TLR4, IL-6 and TNF-α expression were downregulated at transcriptional and post-transcriptional levels.

conclusionPropolis proved to be a promising natural burn healing agent through its antibacterial, antioxidant and anti-inflammatory activities.

Indexed as

Anti-Bacterial AgentsAnti-Inflammatory AgentsAntioxidantsBurnsPropolisStaphylococcus aureusWound HealingAnimalsChromatography, High Pressure LiquidFlavonoidsMaleMiceMicrobial Sensitivity TestsPhytochemicalsAnti-Bacterial AgentsAnti-Inflammatory AgentsAntioxidantsFlavonoidsPhytochemicalsPropolis

Identifiers

PMID38743731
PMCPMC11093344

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.