Evidence map›Paper›PMID 38743369›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

In Vitro Modeling of Feline Morbillivirus Infections Using Primary Feline Kidney Cells.

Michael Sieg, Johannes Busch, Denny Böttcher, Thomas W Vahlenkamp

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Michael SiegFaculty of Veterinary Medicine, Institute of Virology, Leipzig University, Leipzig, Germany. Michael.sieg@mzla.de.ORCID 0000-0003-0799-5787
Johannes BuschFaculty of Veterinary Medicine, Institute of Virology, Leipzig University, Leipzig, Germany.
Denny BöttcherFaculty of Veterinary Medicine, Institute of Veterinary Pathology, Leipzig University, Leipzig, Germany.
Thomas W VahlenkampFaculty of Veterinary Medicine, Institute of Virology, Leipzig University, Leipzig, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Domestic cats are the natural host of feline morbilliviruses (FeMV). Although other species can also be infected (such as dogs and opossums), no laboratory animal infection model is established so far. In vitro models for studying the molecular pathogenesis are therefore needed. For this purpose, propagation and titration of FeMV are key techniques. Unlike other morbilliviruses, such as canine distemper virus (CDV) or measles virus (MV), FeMV is a slow growing virus in cell culture and is difficult to titrate using classical plaque techniques. Here we describe methods for the efficient isolation of FeMV from natural sources (e.g., urine), the propagation of viral stocks, and their titration. In addition, we establish the generation of a three-dimensional infection model mimicking the feline tubular epithelium.

Indexed as

MorbillivirusMorbillivirus InfectionsAnimalsCat DiseasesCatsCells, CulturedDisease Models, AnimalKidneyPrimary Cell CultureVirus CultivationDifferentiated epitheliumFeline morbillivirusPrimary feline kidney cellsVirus isolationVirus quantification

Identifiers

PMID38743369

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.