Evidence map›Paper›PMID 38743362›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Generation of Defective Interfering Particles of Morbilliviruses Using Reverse Genetics.

Linda J Rennick, W Paul Duprex, Natasha L Tilston-Lunel

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Linda J RennickCenter for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
W Paul DuprexCenter for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA. pduprex@pitt.edu.
Natasha L Tilston-LunelDepartment of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA.

Funding

Practices CoreUC7AI180311 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI William Paul Duprex · 2023 to 2026
$13.2M
Defense Advanced Research Projects Agency (DARPA) HR0011940493NIAID NIH HHS UC7 AI180311
6 · The paper itself

Abstract

RNA viruses generate defective genomes naturally during virus replication. Defective genomes that interfere with the infection dynamics either through resource competition or by interferon stimulation are known as defective interfering (DI) genomes. DI genomes can be successfully packaged into virus-like-particles referred to as defective interfering particles (DIPs). Such DIPs can sustainably coexist with the full-length virus particles and have been shown to negatively impact virus replication in vitro and in vivo. Here, we describe a method to generate a clonal DI genome population by reverse genetics. This method is applicable to other RNA viruses and will enable assessment of DIPs for their antiviral properties.

Indexed as

Defective VirusesGenome, ViralMorbillivirusReverse GeneticsVirus ReplicationAnimalsChlorocebus aethiopsHumansRNA, ViralVero CellsVirionRNA, ViralAntiviralsCanine distemper virusDefective genomesDefective interfering particlesMorbillivirusReverse geneticsSingle-step attenuationVaccines

Identifiers

PMID38743362

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.