Evidence map›Paper›PMID 38743359›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

A Semiquantitative Protein-Fragment Complementation Assay to Study Protein-Protein Interactions of the Polymerase Complex in Cellula.

Joanna Brunel, Érica Urzua, Denis Gerlier, Louis-Marie Bloyet

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Joanna BrunelCentre International de Recherche en Infectiologie (CIRI), Inserm U1111, CNRS UMR5308, Université Claude Bernard Lyon1, ENS Lyon, Lyon, France.
Érica UrzuaCentre International de Recherche en Infectiologie (CIRI), Inserm U1111, CNRS UMR5308, Université Claude Bernard Lyon1, ENS Lyon, Lyon, France.
Denis GerlierCentre International de Recherche en Infectiologie (CIRI), Inserm U1111, CNRS UMR5308, Université Claude Bernard Lyon1, ENS Lyon, Lyon, France.
Louis-Marie BloyetCentre International de Recherche en Infectiologie (CIRI), Inserm U1111, CNRS UMR5308, Université Claude Bernard Lyon1, ENS Lyon, Lyon, France. louis-marie.bloyet@inserm.fr.ORCID 0000-0002-5648-3190

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein-fragment complementation assays (PCAs) are powerful tools to investigate protein-protein interactions in a cellular context. These are especially useful to study unstable proteins and weak interactions that may not resist protein isolation or purification. The PCA based on the reconstitution of the Gaussia princeps luciferase (split-luc) is a sensitive approach allowing the mapping of protein-protein interactions and the semiquantitative measurement of binding affinity. Here, we describe the split-luc protocol we used to map the viral interactome of measles virus polymerase complex.

Indexed as

Measles virusProtein BindingProtein Interaction MappingHumansLuciferasesRNA-Dependent RNA PolymeraseViral ProteinsLuciferasesRNA-Dependent RNA PolymeraseViral ProteinsGaussia princeps luciferaseMeasles virus (MeV)NucleoproteinPhosphoproteinPolymerase complexProtein-fragment complementation assay (PCA)Protein-protein interactionRNA-dependent RNA polymeraseSplit-luc reassembly assay

Identifiers

PMID38743359

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.