ArticleMethods in molecular biology (Clifton, N.J.)2024
Proximity Ligation Assay to Detect the Proximity Between Host Proteins and Viral Proteins of HIV-1.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- PAR2-β-arrestin 2-ERK axis mediatesiScience · 2026Article
- An antibody uniquely binding short 2'-O-methyl RNA oligonucleotide duplexes: formation and recognition of target duplexes on cell surfaces.Frontiers in immunology · 2025Article
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5 authors.
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Abstract
The study of host-pathogen interaction often requires interrogating the protein-protein interactions and examining post-translational modifications of the proteins. Traditional protein detection strategies are limited in their sensitivity, specificity, and multiplexing capabilities. The Proximity Ligation Assay (PLA), a versatile and powerful molecular technique, can overcome these limitations. PLA blends the specificity of antibodies, two antibodies detecting two different epitopes on the same or two different proteins, with the amplification efficiency of a polymerase to allow highly specific and sensitive detection of low-abundant proteins, protein-protein interactions, or protein modifications. In this protocol, we describe the application of PLA to detect the proximity between HIV-1 Tat with one of its cellular partners, p65, in an infected host cell. The protocol could be applied to any other context with slight modifications. Of note, PLA can only confirm the physical proximity between two epitopes or proteins; however, the proximity need not necessarily allude to the functional interaction between the two proteins.
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