Evidence map›Paper›PMID 38743226›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Monitoring HIV-1 Nuclear Import Kinetics Using a Chemically Induced Nuclear Pore Blockade Assay.

Adarsh Dharan, Edward M Campbell

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Adarsh DharanDepartment of Biology, Indiana University, Bloomington, IN, USA. adharan@iu.edu.
Edward M CampbellDepartment of Microbiology and Immunology, Stritch School of Medicine, Loyola University, Maywood, Chicago, IL, USA. ecampbell@luc.edu.

Funding

Defining the nuclear import pathways of HIV-1R01AI162694 · NIAID · LOYOLA UNIVERSITY CHICAGO · PI CAMPBELL, EDWARD M · 2021 to 2024
$2.9M
Viral and Cellular Determinants of HIV-1 Nuclear Import KineticsR21AI139009 · NIAID · LOYOLA UNIVERSITY CHICAGO · PI CAMPBELL, EDWARD M · 2018 to 2019
$418k
NIAID NIH HHS R01 AI162694NIAID NIH HHS R21 AI139009
6 · The paper itself

Abstract

To integrate with host chromatin and establish a productive infection, HIV-1 must translocate the viral Ribonucleoprotein (RNP) complex through the nuclear pore complex (NPC). Current assay to measure HIV-1 nuclear import relies on a transient byproduct of HIV-1 integration failure called 2-LTR circles. However, 2-LTR circles require complete or near-complete reverse transcription and association with the non-homologous end joining (NHEJ) machinery in the nucleus, which can complicate interpretation of 2-LTR circle formation as a measure of nuclear import kinetics. Here, we describe an approach to measure nuclear import of infectious HIV-1 particles. This involves chemically induced dimerization of Nup62, a central FG containing nucleoporin. Using this technique, nuclear import of infectious particles can be monitored in both primary and cell culture models. In response to host factor depletion or restriction factors, changes in HIV-1 nuclear import can be effectively measured using the nuclear import kinetics (NIK) assay.

Indexed as

Active Transport, Cell NucleusHIV-1Nuclear PoreNuclear Pore Complex ProteinsCell NucleusHIV InfectionsHumansKineticsVirus IntegrationNuclear Pore Complex Proteins2-LTRHIV-1LentivirusesNuclear importNuclear pore blockadeNuclear pore complexReverse transcription

Identifiers

PMID38743226

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.