Evidence map›Paper›PMID 38743217›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Infectious Virus Tracking by Fluorescent Live Cell Imaging in Primary Cells.

Stephanie Gambut, Thomas J Hope, João I Mamede

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Blockade ofmBio · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Stephanie GambutDepartment of Microbial Pathogens & Immunity, Rush University Medical Center, Chicago, IL, USA.
Thomas J HopeDepartment of Biomedical Engineering, Northwestern University, Chicago, IL, USA.
João I MamedeDepartment of Microbial Pathogens & Immunity, Rush University Medical Center, Chicago, IL, USA. joao_mamede@rush.edu.

Funding

CHEETAH Center for the Structural Biology of HIV Infection, Restriction, and Viral DynamicsU54AI170856 · NIAID · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI WESLEY I. SUNDQUIST · 2022 to 2026
$34.4M
Virus TransmissionP50AI150464 · NIAID · UNIVERSITY OF UTAH · PI HILL, CHRISTOPHER P. · 2019 to 2021
$14.9M
Functionally Defining HIV-Host Interactions During the Early HIV-1 LifecycleR01AI150998 · NIAID · NORTHWESTERN UNIVERSITY AT CHICAGO · PI BIENIASZ, PAUL D., HOPE, THOMAS · 2020 to 2024
$6.8M
HIV innate sensing in glial cells and inflammationR21MH129205 · NIMH · RUSH UNIVERSITY MEDICAL CENTER · PI INACIO MAMEDE, JOAO FILIPE · 2022 to 2023
$467k
Direct live-cell visualization and quantification of interactions between infectious HIV particles in primary target cells.K22AI140963 · NIAID · RUSH UNIVERSITY MEDICAL CENTER · PI INACIO MAMEDE, JOAO FILIPE · 2020 to 2021
$270k
NIAID NIH HHS K22 AI140963NIAID NIH HHS P50 AI150464NIAID NIH HHS R01 AI150998NIAID NIH HHS U54 AI170856NIMH NIH HHS R21 MH129205
6 · The paper itself

Abstract

To successfully infect a cell, HIV-1 has to overcome several host barriers while exploiting cellular cofactors. HIV-1 infection is highly inefficient with the great majority of viral particles not being able to successfully integrate into the target cell genome. Nonproductive HIV-1 particles are degraded or accumulated in cellular compartments. Thus, it becomes hard to distinguish between viral behaviors that lead to effectively infecting the cell from the ones that do not by using traditional methods. Here, we describe the infectious virus tracking method that detects and quantifies individual fluorescent viral particles over time and links viral particle behavior to its infectivity. This method employs live-cell imaging at ultra-low MOIs to detect the outcome of infection for every HIV-1 particle.

Indexed as

HIV-1Cells, CulturedHIV InfectionsHumansMicroscopy, FluorescenceVirionHIV early steps of infectionHIV fusionHIV uncoatingHIV viral coreLive cell imagingWide-field microscopy

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.