ArticleFrontiers in cellular and infection microbiology2024
Raising the bar: genus-specific nested PCR improves detection and lineage identification of avian haemosporidian parasites.
Article in Frontiers in cellular and infection microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Novel Haemoproteus parasite lineage in Hooded Vultures (Necrosyrtes monachus) forms a distinct clade in Accipitriformes and highlights primer selection challenges in understudied avian hosts.Acta parasitologica · 2026Article
- Lineage-specific tissue distribution and high prevalence of haemosporidian parasites in hooded crows (Frontiers in veterinary science · 2026Article
- Survey of haemoprotozoa andInternational journal for parasitology. Parasites and wildlife · 2025Article
- Haemosporidian Infection Risk Variation Across an Urban Gradient in a Songbird.Ecology and evolution · 2025Article
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Authors and funding
7 authors.
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Abstract
Avian haemosporidian parasites are useful model organisms to study the ecology and evolution of parasite-host interactions due to their global distribution and extensive biodiversity. Detection of these parasites has evolved from microscopic examination to PCR-based methods, with the mitochondrial cytochrome b gene serving as barcoding region. However, standard PCR protocols used for screening and identification purposes have limitations in detecting mixed infections and generating phylogenetically informative data due to short amplicon lengths. To address these issues, we developed a novel genus-specific nested PCR protocol targeting avian haemosporidian parasites. The protocol underwent rigorous testing utilizing a large dataset comprising blood samples from Malagasy birds of three distinct Passeriformes families. Furthermore, validation was done by examining smaller datasets in two other laboratories employing divergent master mixes and different bird species. Comparative analyses were conducted between the outcomes of the novel PCR protocol and those obtained through the widely used standard nested PCR method. The novel protocol enables specific identification of
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