ArticleNature communications2024
A cell-free nutrient-supplemented perfusate allows four-day ex vivo metabolic preservation of human kidneys.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Comparison of a New Fully Synthetic Perfusion Solution to Belzer MPS for 24-H Hypothermic Oxygenated Perfusion of Porcine Kidneys: A Feasibility Study.Transplantation direct · 2026Article
- A metabolic test to distinguish temporary and persistent renal graft dysfunction prior to transplantation.Communications medicine · 2026Article
- Alkaline Phosphatase Supplementation During Hypothermic and Normothermic Machine Perfusion Improves Metabolic Dysfunction in a Porcine Model of Donor Kidney Preservation.Transplantation direct · 2026Article
- Comparison of Normothermic and Subnormothermic Machine Perfusion of Porcine Kidneys Using a Novel Fully Synthetic Perfusion Solution: A Proof-of-Concept Study.Journal of clinical medicine · 2026Article
- Cell therapy strategies for organ regeneration.BMC medicine · 2026Review
- Cellular and Molecular Changes Induced by Various Preservation Temperatures and Methods of Preservation in Renal Grafts and Other Solid Organ Grafts.International journal of molecular sciences · 2026Review
- Partial Freezing Enables Functional Preservation of Kidney Grafts for up to 10 Days.Research square · 2026Article
- Revealing the potential of non-transplanted donor organs: a comparative analysis in the Eurotransplant region.Transplant international : official journal of the European Society for Organ Transplantation · 2026Article
- RNA-based therapeutic opportunities for the treatment of kidney diseases.Nature reviews. Nephrology · 2026Review
- Time as a Therapeutic Ally: The Promise of Long-Term Solid Organ and Tissue Perfusion.Transplant international : official journal of the European Society for Organ Transplantation · 2026Review
- Quality Assessment of Discarded Human Kidneys Using Normothermic Perfusion.Transplantation direct · 2026Article
- Improving outcomes in kidney transplantation through advances in donor organ perfusion.Nature reviews. Nephrology · 2025Review
- Progress in monitoring molecular, cellular and metabolic dynamics using mass spectrometry imaging.Bioanalysis · 2025Review
- Systematic production of human kidney organoids for transplantation in porcine kidneys during ex vivo machine perfusion.Nature biomedical engineering · 2025Article
- Beyond preservation: future machine perfusion for liver assessment and repair.Nature reviews. Gastroenterology & hepatology · 2025Review
- Opportunities and challenges with the implementation of normothermic machine perfusion in kidney transplantation.Nature communications · 2025Review
- Normothermic human kidney preservation drives iron accumulation and ferroptosis.Nature communications · 2025Article
- Multifaceted innovations needed to advance transplant nephrology.Nature reviews. Nephrology · 2025Article
- Barriers and Opportunities to Increase Utilization of Donor Kidneys After Death Determined by Circulatory Criteria Among Children and Adults: A Narrative Review.Canadian journal of kidney health and disease · 2025Review
- Organ preservation: current limitations and optimization approaches.Frontiers in medicine · 2025Review
Corrections and comments
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Authors and funding
19 authors.
Funding
Abstract
The growing disparity between the demand for transplants and the available donor supply, coupled with an aging donor population and increasing prevalence of chronic diseases, highlights the urgent need for the development of platforms enabling reconditioning, repair, and regeneration of deceased donor organs. This necessitates the ability to preserve metabolically active kidneys ex vivo for days. However, current kidney normothermic machine perfusion (NMP) approaches allow metabolic preservation only for hours. Here we show that human kidneys discarded for transplantation can be preserved in a metabolically active state up to 4 days when perfused with a cell-free perfusate supplemented with TCA cycle intermediates at subnormothermia (25 °C). Using spatially resolved isotope tracing we demonstrate preserved metabolic fluxes in the kidney microenvironment up to Day 4 of perfusion. Beyond Day 4, significant changes were observed in renal cell populations through spatial lipidomics, and increases in injury markers such as LDH, NGAL and oxidized lipids. Finally, we demonstrate that perfused kidneys maintain functional parameters up to Day 4. Collectively, these findings provide evidence that this approach enables metabolic and functional preservation of human kidneys over multiple days, establishing a solid foundation for future clinical investigations.
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