Evidence map›Paper›PMID 38740717›Full record

ArticleBiotechnology letters2024

Essential amino acid residues and catalytic mechanism of trans-epoxysuccinate hydrolase for production of meso-tartaric acid.

Hongxiu Liao, Haifeng Pan, Jinfeng Yao, Ronglin Zhu, Wenna Bao

Abstract read
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In one paragraph

Article in Biotechnology letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Improving the thermal stability ofJournal of Zhejiang University. Science. B · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Hongxiu Liao *School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China.
Haifeng Pan *Huzhou College, Huzhou, 313000, China.
Jinfeng YaoSchool of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China.
Ronglin ZhuSchool of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China.
Wenna BaoSchool of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China. wennabao@163.com.ORCID http://orcid.org/0000-0003-3452-7938

Funding

Huzhou Scientific and Technological Project 2022GZ56the Education of Zhejiang Province of China Y202248484
6 · The paper itself

Abstract

objectivesThis study aimed to discuss the essential amino acid residues and catalytic mechanism of trans-epoxysuccinate hydrolase from Pseudomonas koreensis for the production of meso-tartaric acid.

resultsThe optimum conditions of the enzyme were 45 °C and pH 9.0, respectively. It was strongly inhibited by Zn

conclusionsThe structure and catalytic mechanism of trans-epoxysuccinate hydrolase were first reported. Ten residues were critical for its catalysis and a two-step mechanism by an Asp-His-Asp catalytic triad was proposed.

Indexed as

PseudomonasTartratesBacterial ProteinsCatalysisHydrogen-Ion ConcentrationHydrolasesKineticsModels, MolecularMutagenesis, Site-DirectedBacterial ProteinsHydrolasestartaric acidTartratesCatalytic mechanismMeso-tartaric acidPseudomonas koreensisTrans-epoxysuccinate hydrolase

Identifiers

PMID38740717

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.