Evidence map›Paper›PMID 38737670›Full record

ArticleIsrael journal of chemistry2023

Chemoenzymatic Tagging of Tn/TF/STF Antigens in Living Systems.

Yi Yang, Mingkuan Chen, Mengyao Wu, Senlian Hong, Bing Gao, Yonghui Liu, Chenhua Yu, Travis S Young, Digantkumar Gopaldas Chapla, Jeong-Yeh Yang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Israel journal of chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yi YangDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Mingkuan ChenDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Mengyao WuDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Senlian HongDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Bing GaoDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Yonghui LiuDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Chenhua YuDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Travis S YoungDepartment of Biology, California Institute for Biomedical Research (Calibr), The Scripps Research Institute, La Jolla, CA 92037, USA.
Digantkumar Gopaldas ChaplaDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602.
Jeong-Yeh YangDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602.
John R CappielloDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Jie P LiDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
K Barry SharplessDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Kelley W MoremenDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602.
Peng WuDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.

Funding

TR&D3. Approaches to Proteoglycan Function at Biochemical and Cellular LevelsP41GM103390 · NIGMS · UNIVERSITY OF GEORGIA · PI MOREMEN, KELLEY W. · 2012 to 2019
$14.5M
Recombinant Production of Glycosylation Enzymes for Biochem/Structural StudiesP01GM107012 · NIGMS · UNIVERSITY OF GEORGIA · PI MOREMEN, KELLEY · 2013 to 2017
$7.5M
Chemoenzymatic glycan editing for deciphering biological functions of glycansR35GM139643 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI Peng Wu · 2021 to 2026
$4.7M
Sulfur(VI) Fluoride Exchange (SuFEx): new developments and biological applicationsR01GM117145 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI SHARPLESS, KARL BARRY · 2016 to 2023
$3.9M
Origin of N-Glycan Site-Specific HeterogeneityR01GM130915 · NIGMS · UNIVERSITY OF GEORGIA · PI KANNAN, NATARAJAN, MOREMEN, KELLEY W. · 2019 to 2022
$3.4M
Streamlining the chemoenzymatic synthesis of asymmetrical glycans of biological importanceU01GM120408 · NIGMS · UNIVERSITY OF GEORGIA · PI BOONS, GEERT-JAN · 2016 to 2019
$3.0M
NIGMS NIH HHS P01 GM107012NIGMS NIH HHS P41 GM103390NIGMS NIH HHS R01 GM117145NIGMS NIH HHS R01 GM130915NIGMS NIH HHS R35 GM139643NIGMS NIH HHS U01 GM120408
6 · The paper itself

Abstract

Truncated mucin-type O-glycans, such as Tn-associated antigens, are aberrantly expressed biomarkers of cancer, but remain challenging to target. Reactive antibodies to these antigens either lack high-affinity or are prone to antigen escape. Here, we have developed a robust chemoenzymatic strategy for the global labeling of Tn-associated antigens, i.e. Tn (GalNAcα-O-Ser/Thr), Thomsen-Friedenreich (Galβ1-3GalNAcα-O-Ser/Thr, TF) and STF (Neu5Acα2-3Galβ1-3GalNAcα-O-Ser/Thr, STF) antigens, in human whole blood with high efficiency and selectivity. This method relies on the use of the O-glycan sialyltransferase ST6GalNAc1 to transfer a sialic acid-functionalized adaptor to the GalNAc residue of these antigens. By tagging, the adaptor functionalized antigens can be easily targeted by customized strategies such as, but not limited to, chimeric antigen receptor T-Cells (CAR-T). We expect this tagging system to find broad applications in cancer diagnostics and targeting in combination with established strategies.

Indexed as

CAR-TChemoenzymatic glycan editingTn-associated antigens

Identifiers

PMID38737670
PMCPMC11081099

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.