Evidence map›Paper›PMID 38733425›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2024

Rapid colorimetric polymyxin B microelution directly from positive blood bottles: because patients with serious infections should not have to wait for results of culture-based methodologies.

Gabriela da Silva Collar, Julia Becker, Natália Kehl Moreira, Luana Silva Dornelles, Mariana Preussler Mott, Afonso Luís Barth, Juliana Caierão

Abstract readEvaluation Study
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In one paragraph

Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Gabriela da Silva CollarLaboratório de Pesquisa em Bacteriologia Clínica (LaBaC), Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. gabi.collar@gmail.com.ORCID http://orcid.org/0000-0003-2819-0769
Julia BeckerLaboratório de Pesquisa em Bacteriologia Clínica (LaBaC), Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Natália Kehl MoreiraLaboratório de Pesquisa em Bacteriologia Clínica (LaBaC), Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Luana Silva DornellesLaboratório de Microbiologia Clínica, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Mariana Preussler MottLaboratório de Microbiologia Clínica, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Afonso Luís BarthLaboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Juliana CaierãoLaboratório de Pesquisa em Bacteriologia Clínica (LaBaC), Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.ORCID http://orcid.org/0000-0002-8776-8447

Funding

Fundo de Incentivo à Pesquisa e Eventos from Hospital de Clínicas de Porto Alegre - FIPE/ HCPA 2023-0061Instituto Nacional de Pesquisa em Resistência Antimicrobiana - INPRA 465718/2014-0
6 · The paper itself

Abstract

purposeTo evaluate the performance of the rapid colorimetric polymyxin B microelution (RCPEm) in determining polymyxin B resistance directly from Enterobacterales-positive blood cultures.

methodsA set volume of positive blood culture bottles (diluted 1:10) was inoculated into a glucose-broth-phenol red solution (NP solution), where a polymyxin B disk was previously eluted (final concentration of 3 µg/mL). Test was read each 1 h for up to 4 h. Color change from red/orange to yellow indicated resistant isolates. Results were compared to the reference method, broth microdilution (BMD), performed from colonies grown on solid media from the same blood culture bottle.

resultsOne hundred fifty-two Enterobacterales-positive blood cultures were evaluated, 22.4% (34/152) of them resistant to polymyxin B (including 6.6% with borderline MICs). When performing directly from positive blood cultures (RCPEm-BC), specificity and sensitivity were 99.1% and 94.1%, respectively. Of note, 79.4% (27/34) of truly resistant isolates required 3 h of incubation, compared to the 18 ± 2 h incubation that microtiter plates of BMD demand before reading can be performed.

conclusionsRCPEm directly from blood cultures has great potential to be part of the routine of clinical microbiology laboratories to establish polymyxin B susceptibility, impacting outcome of patients with bloodstream infections caused by carbapenem-resistant Enterobacterales.

Indexed as

Anti-Bacterial AgentsBlood CultureColorimetryMicrobial Sensitivity TestsPolymyxin BBacteremiaDrug Resistance, BacterialEnterobacteriaceaeEnterobacteriaceae InfectionsHumansSensitivity and SpecificityAnti-Bacterial AgentsPolymyxin BBlood cultureColorimetric testEnterobacteralesPolymyxin BPolymyxinsRCPEm

Identifiers

PMID38733425

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.