Evidence map›Paper›PMID 38733093›Full record

ArticlePlant biotechnology journal2024

Protein complexes from edible mushrooms as a sustainable potato protection against coleopteran pests.

Karmen Pogačar, Maja Grundner, Primož Žigon, Anna Coll, Anastasija Panevska, Tjaša Lukan, Marko Petek, Jaka Razinger, Kristina Gruden, Kristina Sepčić

Abstract read
In one paragraph

Article in Plant biotechnology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Karmen PogačarDepartment of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.ORCID 0000-0001-5190-7217
Maja GrundnerDepartment of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Primož ŽigonPlant Protection Department, Agricultural Institute of Slovenia, Ljubljana, Slovenia.
Anna CollDepartment of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.ORCID 0000-0003-0672-1530
Anastasija PanevskaDepartment of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Tjaša LukanDepartment of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.ORCID 0000-0002-6235-2816
Marko PetekDepartment of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.ORCID 0000-0003-3644-7827
Jaka RazingerPlant Protection Department, Agricultural Institute of Slovenia, Ljubljana, Slovenia.
Kristina GrudenDepartment of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.ORCID 0000-0001-5906-8569
Kristina SepčićDepartment of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.ORCID 0000-0002-4023-5379

Funding

Javna Agencija za Raziskovalno Dejavnost RS J4-1772Javna Agencija za Raziskovalno Dejavnost RS P1-0207Javna Agencija za Raziskovalno Dejavnost RS P4-0165
6 · The paper itself

Abstract

Protein complexes from edible oyster mushrooms (Pleurotus sp.) composed of pleurotolysin A2 (PlyA2) and pleurotolysin B (PlyB) exert toxicity in feeding tests against Colorado potato beetle (CPB) larvae, acting through the interaction with insect-specific membrane sphingolipid. Here we present a new strategy for crop protection, based on in planta production of PlyA2/PlyB protein complexes, and we exemplify this strategy in construction of transgenic potato plants of cv Désirée. The transgenics in which PlyA2 was directed to the vacuole and PlyB to the endoplasmic reticulum are effectively protected from infestation by CPB larvae without impacting plant performance. These transgenic plants showed a pronounced effect on larval feeding rate, the larvae feeding on transgenic plants being on average five to six folds lighter than larvae feeding on controls. Further, only a fraction (11%-37%) of the larvae that fed on transgenic potato plants completed their life cycle and developed into adult beetles. Moreover, gene expression analysis of CPB larvae exposed to PlyA2/PlyB complexes revealed the response indicative of a general stress status of larvae and no evidence of possibility of developing resistance due to the functional inactivation of PlyA2/PlyB sphingolipid receptors.

Indexed as

ColeopteraLarvaPlants, Genetically ModifiedSolanum tuberosumAgaricalesAnimalsFungal ProteinsPest Control, BiologicalFungal Proteinsbiopesticidesinsect stress responselarval toxicityLeptinotarsa decemlineataSolanum tuberosumtransgenic crop

Identifiers

PMID38733093
PMCPMC11331795

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.