Evidence map›Paper›PMID 38731834›Full record

ArticleInternational journal of molecular sciences2024

TRIM44 Promotes Rabies Virus Replication by Autophagy-Dependent Mechanism.

Hongling He, Ting Cai, Qiaozhu Chen, Zilian Chen, Boyue Zhang, Changyi Chen, Yueze Wang, Yan Liu, Yueming Wang, Yongwen Luo and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Hongling HeCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
Ting CaiCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
Qiaozhu ChenCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
Zilian ChenCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
Boyue ZhangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.ORCID 0009-0008-5569-4261
Changyi ChenCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
Yueze WangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
Yan LiuCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
Yueming WangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
Yongwen LuoCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.
Shile HuangDepartment of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130-3932, USA.ORCID 0000-0002-3239-1072
Jun LuoCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.ORCID 0000-0003-4422-9619
Xiaofeng GuoCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510000, China.ORCID 0000-0003-2896-3629

Funding

the Basic and Applied Basic Research Project of Guangzhou Basic Research Program No. 202201010652the Double First-class Discipline Promotion Project No. 2023B10564003)the National Nature Science Foundation of China No. 32002270the Nature Science Foundation of Guangdong No.2022A1515012530This study was partially supported by the National Key Research and Development Plan 2022YFD1800100
6 · The paper itself

Abstract

Tripartite motif (TRIM) proteins are a multifunctional E3 ubiquitin ligase family that participates in various cellular processes. Recent studies have shown that TRIM proteins play important roles in regulating host-virus interactions through specific pathways, but their involvement in response to rabies virus (RABV) infection remains poorly understood. Here, we identified that several TRIM proteins are upregulated in mouse neuroblastoma cells (NA) after infection with the rabies virus using RNA-seq sequencing. Among them, TRIM44 was found to regulate RABV replication. This is supported by the observations that downregulation of TRIM44 inhibits RABV replication, while overexpression of TRIM44 promotes RABV replication. Mechanistically, TRIM44-induced RABV replication is brought about by activating autophagy, as inhibition of autophagy with 3-MA attenuates TRIM44-induced RABV replication. Additionally, we found that inhibition of autophagy with rapamycin reverses the TRIM44-knockdown-induced decrease in LC3B expression and autophagosome formation as well as RABV replication. The results suggest that TRIM44 promotes RABV replication by an autophagy-dependent mechanism. Our work identifies TRIM44 as a key host factor for RABV replication, and targeting TRIM44 expression may represent an effective therapeutic strategy.

Indexed as

AutophagyRabies virusTripartite Motif ProteinsVirus ReplicationAnimalsCell Line, TumorHost-Pathogen InteractionsHumansIntracellular Signaling Peptides and ProteinsMiceRabiesIntracellular Signaling Peptides and ProteinsTripartite Motif ProteinsautophagyE3 ubiquitin ligaserabies virusreplicationTRIM44

Identifiers

PMID38731834
PMCPMC11083291

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.