Evidence map›Paper›PMID 38730257›Full record

ArticleNature biomedical engineering2024

Imaging bioluminescence by detecting localized haemodynamic contrast from photosensitized vasculature.

Robert Ohlendorf, Nan Li, Valerie Doan Phi Van, Miriam Schwalm, Yuting Ke, Miranda Dawson, Ying Jiang, Sayani Das, Brenna Stallings, Wen Ting Zheng and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biomedical engineering, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Robert Ohlendorf *Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-1720-4400
Nan Li *Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Valerie Doan Phi VanDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Miriam SchwalmDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Yuting KeDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-0755-0737
Miranda DawsonDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-3226-9122
Ying JiangDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Sayani DasDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-0206-9223
Brenna StallingsDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0001-7677-0261
Wen Ting ZhengDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Alan JasanoffDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. jasanoff@mit.edu.ORCID http://orcid.org/0000-0002-2834-6359

Funding

Toward functional molecular neuroimaging using vasoactive probes in human subjectsU01EB031641 · NIBIB · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI JASANOFF, ALAN · 2021 to 2025
$2.9M
Analysis of Integrated Brain Functions Using Hemogenetic ImagingR01NS121073 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Alan Jasanoff · 2022 to 2026
$2.6M
Hemogenetic imaging technology for circuit-specific analysis of primate brain functionUG3MH126868 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI JASANOFF, ALAN · 2021 to 2023
$1.8M
Multimodal probes for multiscale calcium imagingR21EY032369 · NEI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI JASANOFF, ALAN · 2021 to 2022
$464k
NEI NIH HHS R21 EY032369NIBIB NIH HHS U01 EB031641NIMH NIH HHS UG3 MH126868NINDS NIH HHS R01 NS121073
6 · The paper itself

Abstract

Bioluminescent probes are widely used to monitor biomedically relevant processes and cellular targets in living animals. However, the absorption and scattering of visible light by tissue drastically limit the depth and resolution of the detection of luminescence. Here we show that bioluminescent sources can be detected with magnetic resonance imaging by leveraging the light-mediated activation of vascular cells expressing a photosensitive bacterial enzyme that causes the conversion of bioluminescent emission into local changes in haemodynamic contrast. In the brains of rats with photosensitized vasculature, we used magnetic resonance imaging to volumetrically map bioluminescent xenografts and cell populations virally transduced to express luciferase. Detecting bioluminescence-induced haemodynamic signals from photosensitized vasculature will extend the applications of bioluminescent probes.

Indexed as

HemodynamicsLuminescent MeasurementsMagnetic Resonance ImagingAnimalsBrainHumansLuciferasesMiceRatsLuciferases

Identifiers

PMID38730257
PMCPMC12121613

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.