Evidence map›Paper›PMID 38730188›Full record

ArticleAMB Express2024

Enzyme variants in biosynthesis and biological assessment of different molecular weight hyaluronan.

Tahereh Ebrahimi, Malihe Keramati, Farnaz Khodabakhsh, Reza Ahangari Cohan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AMB Express, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Hyaluronic Acid and Its Synthases-Current Knowledge.International journal of molecular sciences · 2025
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tahereh EbrahimiNew Technologies Research Group, Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.ORCID http://orcid.org/0000-0002-3457-0082
Malihe KeramatiNew Technologies Research Group, Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran. mkeramati189@gmail.com.ORCID http://orcid.org/0000-0002-7196-8751
Farnaz KhodabakhshDepartment of Genetics and Advanced Medical Technology, Faculty of Medicine, Medical Biotechnology Research Center, AJA University of Medical Sciences, Tehran, Iran.ORCID http://orcid.org/0000-0002-3367-5288
Reza Ahangari CohanNew Technologies Research Group, Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran. cohan.reza@gmail.com.ORCID http://orcid.org/0000-0002-0490-4184

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present study, low- and high-molecular-weight hyaluronic acids (LMW-HA and HMW-HA) were synthesized in vitro by truncated Streptococcus equisimilis hyaluronan synthases (SeHAS). The enzyme kinetic parameters were determined for each enzyme variant. The MW, structure, dispersity, and biological activity of polymers were determined by electrophoresis, FTIR spectroscopy, carbazole, cell proliferation, and cell migration assay, respectively. The specific activities were calculated as 7.5, 6.8, 4.9, and 2.8 µg

Indexed as

Hyaluronan synthaseHyaluronic acidMolecular weightPolydispersitySpecific activity

Identifiers

PMID38730188
PMCPMC11087452

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.