Evidence map›Paper›PMID 38727863›Full record

ArticleMolecular biology reports2024

CircCOX6A1 suppresses osteogenic differentiation and aggravates osteoporosis via miR-512-3p/DYRK2 axis.

Dingwen He, Sikuan Zheng, Jian Cao, Jianjian Deng, Rui Ding, Yanjie Xu, Xigao Cheng

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Dingwen HeDepartment of Orthopaedics, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, Nanchang, Jiangxi, 330006, China.
Sikuan ZhengSchool of Medical College, Nanchang University, Nanchang, Jiangxi, 330006, China.
Jian CaoDepartment of Orthopaedics, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, Nanchang, Jiangxi, 330006, China.
Jianjian DengDepartment of Orthopaedics, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, Nanchang, Jiangxi, 330006, China.
Rui DingSchool of Medical College, Nanchang University, Nanchang, Jiangxi, 330006, China.
Yanjie XuDepartment of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, Nanchang, Jiangxi, 330006, China. 745950710@qq.com.
Xigao ChengDepartment of Orthopaedics, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, Nanchang, Jiangxi, 330006, China. chengxigao_edu@outlook.com.

Funding

Jiangxi Provincial Administration of Traditional Chinese Medicine Science and Technology Plan 2022B985
6 · The paper itself

Abstract

backgroundOsteoporosis (OP), characterized by compromised bone integrity and increased fracture risk, poses a significant health challenge. Circular RNAs (circRNAs) have emerged as crucial regulators in various pathophysiological processes, prompting investigation into their role in osteoporosis. This study aimed to elucidate the involvement of circCOX6A1 in OP progression and understand its underlying molecular mechanisms. The primary objective was to explore the impact of circCOX6A1 on bone marrow-derived mesenchymal stem cells (BMSCs) and its potential interactions with miR-512-3p and DYRK2.

methodsGSE161361 microarray analysis was employed to assess circCOX6A1 expression in OP patients. We utilized in vitro and in vivo models, including BMSC cultures, osteogenic differentiation assays, and an OVX-induced mouse model of OP. Molecular techniques such as quantitative RT-PCR, western blotting, and functional assays like alizarin red staining (ARS) were employed to evaluate circCOX6A1 effects on BMSC proliferation, apoptosis, and osteogenic differentiation. The interaction between circCOX6A1, miR-512-3p, and DYRK2 was investigated through dual luciferase reporter assays, RNA immunoprecipitation, and RNA pull-down assays.

resultsCircCOX6A1 was found to be upregulated in osteoporosis patients, and its expression inversely correlated with osteogenic differentiation of BMSCs. CircCOX6A1 knockdown enhanced osteogenic differentiation, as evidenced by increased mineralized nodule formation and upregulation of osteogenic markers. In vivo, circCOX6A1 knockdown ameliorated osteoporosis progression in OVX mice. Mechanistically, circCOX6A1 acted as a sponge for miR-512-3p, subsequently regulating DYRK2 expression.

conclusionThis study provides compelling evidence for the role of circCOX6A1 in osteoporosis pathogenesis. CircCOX6A1 negatively regulates BMSC osteogenic differentiation through the miR-512-3p/DYRK2 axis, suggesting its potential as a therapeutic target for mitigating OP progression.

Indexed as

Cell DifferentiationDyrk KinasesMesenchymal Stem CellsMicroRNAsOsteogenesisOsteoporosisProtein Serine-Threonine KinasesProtein-Tyrosine KinasesRNA, CircularAnimalsApoptosisCell ProliferationDisease Models, AnimalMiceDyrk2 protein, mouseDyrk KinasesMicroRNAsProtein Serine-Threonine KinasesProtein-Tyrosine KinasesRNA, CircularcircCOX6A1DYRK2miR-512-3pOsteogenic differentiationOsteoporosis

Identifiers

PMID38727863

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.