Evidence map›Paper›PMID 38727318›Full record

ArticleCells2024

Comprehensive Analysis of CXCR4, JUNB, and PD-L1 Expression in Circulating Tumor Cells (CTCs) from Prostate Cancer Patients.

Argyro Roumeliotou, Areti Strati, Foteini Chamchougia, Anastasia Xagara, Victoria Tserpeli, Stavroula Smilkou, Elina Lagopodi, Athina Christopoulou, Emmanouil Kontopodis, Ioannis Drositis and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
  6. JUNB and JUND in Urological Cancers: A Literature Review.Current issues in molecular biology · 2025
    Review
  7. Review
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Argyro RoumeliotouLaboratory of Biochemistry/Metastatic Signaling, Department of Biology, University of Patras, 26504 Patras, Greece.
Areti StratiAnalysis of Circulating Tumor Cells Lab, Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece.ORCID 0000-0001-9644-5538
Foteini ChamchougiaLaboratory of Biochemistry/Metastatic Signaling, Department of Biology, University of Patras, 26504 Patras, Greece.
Anastasia XagaraFaculty of Medicine, School of Health Sciences, University of Thessaly, 41500 Larissa, Greece.ORCID 0000-0001-8104-1514
Victoria TserpeliAnalysis of Circulating Tumor Cells Lab, Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece.
Stavroula SmilkouAnalysis of Circulating Tumor Cells Lab, Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece.ORCID 0000-0002-5338-0276
Elina LagopodiAnalysis of Circulating Tumor Cells Lab, Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece.
Athina ChristopoulouOncology Unit, ST Andrews General Hospital of Patras, 26332 Patras, Greece.
Emmanouil KontopodisDepartment of Oncology, Venizeleion General Hospital of Heraklion, 71409 Heraklion, Greece.
Ioannis DrositisDepartment of Oncology, Venizeleion General Hospital of Heraklion, 71409 Heraklion, Greece.
Nikolaos AndroulakisDepartment of Oncology, Venizeleion General Hospital of Heraklion, 71409 Heraklion, Greece.
Vassilis GeorgouliasHellenic Oncology Research Group, 11526 Athens, Greece.
Filippos KoinisFaculty of Medicine, School of Health Sciences, University of Thessaly, 41500 Larissa, Greece.
Athanasios KotsakisFaculty of Medicine, School of Health Sciences, University of Thessaly, 41500 Larissa, Greece.ORCID 0000-0002-5530-6774
Evi LianidouAnalysis of Circulating Tumor Cells Lab, Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece.ORCID 0000-0002-7796-5914
Galatea KallergiLaboratory of Biochemistry/Metastatic Signaling, Department of Biology, University of Patras, 26504 Patras, Greece.ORCID 0000-0001-9825-3871

Funding

EU NextGenEU through the General Secretariat for Research and Innovation of the Hellenic Ministry of Development as part of the Hellenic Precision Medicine Network (ΗPMN) (MIS) 5184864European Regional Development Fund of the European Union and Greek funds through the Op-erational Program Competitiveness Entrepreneurship and Innovation, under the call RESEARCH-CREATE-INNOVATE T2EDK-01562
6 · The paper itself

Abstract

CXCR4, JUNB and PD-L1 are implicated in cancer progression and metastasis. The current study investigated these biomarkers in CTCs isolated from metastatic prostate cancer (mPCa) patients at the RNA and protein levels. CTCs were isolated from 48 mPCa patients using the Ficoll density gradient and ISET system (17 out of 48). The (CK/PD-L1/CD45) and (CK/CXCR4/JUNB) phenotypes were identified using two triple immunofluorescence stainings followed by VyCAP platform analysis. Molecular analysis was conducted with an EpCAM-dependent method for 25/48 patients.

Indexed as

B7-H1 AntigenNeoplastic Cells, CirculatingProstatic NeoplasmsReceptors, CXCR4AgedBiomarkers, TumorGene Expression Regulation, NeoplasticHumansMaleMiddle AgedProto-Oncogene Proteins c-junTranscription FactorsB7-H1 AntigenBiomarkers, TumorCD274 protein, humanCXCR4 protein, humanJunB protein, humanProto-Oncogene Proteins c-junReceptors, CXCR4Transcription Factorscirculating tumor cellsCXCR4JUNBmRNAPD-L1prostate cancer

Identifiers

PMID38727318
PMCPMC11083423

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.