ArticlePhotoacoustics2024
On-demand expansion fluorescence and photoacoustic microscopy (ExFLPAM).
Article in Photoacoustics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Label-free ultraviolet photoacoustic microscopy for multiscale structural phenotyping of epithelial morphogenesis in organoid-derived intestinal epithelial wrinkle tissue.Photoacoustics · 2026Article
- Activatable smart contrast agents for photoacoustic imaging.Smart molecules : open access · 2026Review
- Fabrication of optically transparent ultrasound transducers to integrate light and sound in multimodal biomedical systems.Nature protocols · 2026Review
- Volumetric photoacoustic/ultrasound image fusion and enhancement with dual-tree complex wavelet transform.Biomedical optics express · 2026Article
- Photoacoustic Microscopy for Multiscale Biological System Visualization and Clinical Translation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- From light to sound: Seeing and hearing the placenta in health and disease.Science advances · 2026Review
- A Hybrid Diffusion Model Enhances Multiparametric 3D Photoacoustic Computed Tomography.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A handheld photoacoustic microscopic probe integrating a transparent ultrasound transducer and a fiber scanner.Nature communications · 2025Article
- Virtual-point-based deconvolution for optical-resolution photoacoustic microscopy.Journal of biophotonics · 2024Article
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9 authors.
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Abstract
Expansion microscopy (ExM) is a promising technology that enables nanoscale imaging on conventional optical microscopes by physically magnifying the specimens. Here, we report the development of a strategy that enables i) on-demand labeling of subcellular organelles in live cells for ExM through transfection of fluorescent proteins that are well-retained during the expansion procedure; and ii) non-fluorescent chromogenic color-development towards efficient bright-field and photoacoustic imaging in both planar and volumetric formats, which is applicable to both cultured cells and biological tissues. Compared to the conventional ExM methods, our strategy provides an expanded toolkit, which we term as expansion fluorescence and photoacoustic microscopy (ExFLPAM), by allowing on-demand fluorescent protein labeling of cultured cells, as well as non-fluorescent absorption contrast-imaging of biological samples.
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