ArticleMatters select2017
Effective Generation of Gynogenic Haploid Zebrafish Embryos Using Low Dosage of UV Rays.
Article in Matters select, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
3 authors.
Funding
Abstract
The phenomenon of phenotype manifestation when the single allele in a haploid is affected is desirable for uncovering recessive mutations expeditiously in a diploid organism. However, experimentally generated haploids manifest extensive lethality and a cluster of non-specific developmental defects known as the haploid syndrome. This precludes the use of experimentally generated haploids for genetic screens due to an insufficient number of embryos for screening and the possibility of phenotypes due to the affected gene being masked by the haploid syndrome. We show here that gynogenic haploid zebrafish can be generated by irradiation of spermatozoa with a lower UV dosage than is currently used. This strategy results in reduced haploid lethality, incidence and severity of haploid syndrome. When viewed in the context of zebrafish as a genetically tractable model organism for forward and reverse genetic strategies, these results place zebrafish in a unique niche as a vertebrate in which haploid genetic screens for developmental phenotypes could be successfully attempted.
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