Evidence map›Paper›PMID 38725539›Full record

ArticleMediators of inflammation2024

Sorbitol Destroyed Intestinal Microfold Cells (M Cells) Development through Inhibition of PDE4-Mediated RANKL Expression.

Li Xiang, Wenxu Pan, Huan Chen, Wenjun Du, Shuping Xie, Xinhua Liang, Fangying Yang, Rongwei Niu, Canxin Huang, Minan Luo and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mediators of inflammation, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

15 authors.

Li XiangGuangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Wenxu PanDepartment of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.
Huan ChenDepartment of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.
Wenjun DuDepartment of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.
Shuping XieGuangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Xinhua LiangDepartment of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.
Fangying YangDepartment of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.
Rongwei NiuThe First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China.
Canxin HuangThe Second Clinical Medical School, Guangzhou Medical University, Guangzhou, China.
Minan LuoThe School of Pediatrics, Guangzhou Medical University, Guangzhou, China.
Yuxin XuGuangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Lanlan GengGuangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Sitang GongGuangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Wanfu XuGuangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.ORCID https://orcid.org/0000-0002-1467-9830
Junhong ZhaoDepartment of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.ORCID https://orcid.org/0000-0002-3302-4979

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: Microfold cells (M cells) are specific intestinal epithelial cells for monitoring and transcytosis of antigens, microorganisms, and pathogens in the intestine. However, the mechanism for M-cell development remained elusive. Materials and Methods: Real-time polymerase chain reaction, immunofluorescence, and western blotting were performed to analyze the effect of sorbitol-regulated M-cell differentiation Results: Herein, in comparison to the mannitol group (control group), we found that intestinal M-cell development was inhibited in response to sorbitol treatment as evidenced by impaired enteroids accompanying with decreased early differentiation marker Annexin 5, Marcksl1, Spib, sox8, and mature M-cell marker glycoprotein 2 expression, which was attributed to downregulation of receptor activator of nuclear factor kappa-В ligand (RANKL) expression Conclusion: These findings suggested that sorbitol suppressed intestinal enteroids and M-cell differentiation and matured through PDE4-mediated RANKL expression; targeting to inhibit PDE4 was sufficient to induce M-cell development.

Indexed as

Cyclic AMP Response Element-Binding ProteinCyclic Nucleotide Phosphodiesterases, Type 4M CellsRANK LigandSorbitolAnimalsCell DifferentiationCyclic AMP-Dependent Protein KinasesIntestinal MucosaMaleMiceMice, Inbred C57BLCyclic AMP-Dependent Protein KinasesCyclic AMP Response Element-Binding ProteinCyclic Nucleotide Phosphodiesterases, Type 4RANK LigandSorbitol

Identifiers

PMID38725539
PMCPMC11081746

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.