Evidence map›Paper›PMID 38725139›Full record

ArticleJournal of cachexia, sarcopenia and muscle2024

Inflammation-associated intramyocellular lipid alterations in human pancreatic cancer cachexia.

Min Deng, Jianhua Cao, Gregory van der Kroft, David P J van Dijk, Merel R Aberle, Andrej Grgic, Ulf P Neumann, Georg Wiltberger, Benjamin Balluff, Frank G Schaap and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cachexia, sarcopenia and muscle, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Min DengDepartment of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.ORCID 0000-0002-9337-3935
Jianhua CaoDivision of Imaging Mass Spectrometry, Maastricht Multimodal Molecular Imaging (M4i) Institute, Maastricht University, Maastricht, The Netherlands.
Gregory van der KroftDepartment of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.
David P J van DijkDepartment of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.
Merel R AberleDepartment of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.
Andrej GrgicDivision of Imaging Mass Spectrometry, Maastricht Multimodal Molecular Imaging (M4i) Institute, Maastricht University, Maastricht, The Netherlands.
Ulf P NeumannDepartment of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.
Georg WiltbergerDepartment of General, Visceral and Transplantation Surgery, RWTH University Hospital Aachen, Aachen, Germany.
Benjamin BalluffDivision of Imaging Mass Spectrometry, Maastricht Multimodal Molecular Imaging (M4i) Institute, Maastricht University, Maastricht, The Netherlands.
Frank G SchaapDepartment of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.
Ron M A HeerenDivision of Imaging Mass Spectrometry, Maastricht Multimodal Molecular Imaging (M4i) Institute, Maastricht University, Maastricht, The Netherlands.
Steven W M Olde DaminkDepartment of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.
Sander S RensenDepartment of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.ORCID 0000-0001-5054-8400

Funding

China Scholarship Council 201806820029
6 · The paper itself

Abstract

backgroundCancer cachexia is a multifactorial metabolic syndrome characterized by systemic inflammation and ongoing skeletal muscle loss resulting in weakness, poor quality of life, and decreased survival. Whereas lipid accumulation in skeletal muscle is associated with cancer cachexia as well as the prognosis of cancer patients, surprisingly little is known about the nature of the lipids that accumulate in the muscle during cachexia, and whether this is related to inflammation. We aimed to identify the types and distributions of intramyocellular lipids in patients with and without cancer cachexia.

methodsRectus abdominis muscle biopsies were collected during surgery of patients with pancreatic ductal adenocarcinoma (n = 10 without cachexia, n = 20 cachectic without inflammation (CRP < 10 mg/L), n = 10 cachectic with inflammation (CRP ≥ 10 mg/L). L3-CT scans were analysed to assess body composition based on validated thresholds in Hounsfield units (HU). Muscle sections were stained with Oil-Red O and H&E to assess general lipid accumulation and atrophy. Untargeted lipidomic analyses were performed on laser-microdissected myotubes using LC-MS/MS. The spatial distribution of intramyocellular lipids with differential abundance between groups was visualized by mass-spectrometry imaging. Genes coding for inflammation markers and enzymes involved in de novo ceramide synthesis were studied by qPCR.

resultsMuscle radiation attenuation was lower in cachectic patients with inflammation (median 24.3 [18.6-30.8] HU) as compared with those without inflammation (34.2 [29.3-38.7] HU, P = 0.033) or no cachexia (37.4 [33.9-42.9] HU, P = 0.012). Accordingly, intramyocellular lipid content was lower in non-cachectic patients (1.9 [1.6-2.1]%) as compared with those with cachexia with inflammation (5.5 [4.5-7.3]%, P = 0.002) or without inflammation (4.8 [2.6-6.0]%, P = 0.017). Intramyocellular lipid accumulation was associated with both local IL-6 mRNA levels (r

conclusionsIntramyocellular lipid accumulation in patients with cachexia is associated with both local and systemic inflammation, and characterized by changes in defined lipid species such as glycerolipids and glycerophospholipids.

Indexed as

CachexiaInflammationLipid MetabolismPancreatic NeoplasmsAgedBody CompositionFemaleHumansLipidsMaleMiddle AgedMuscle, SkeletalLipidsCancer cachexiaCeramidesIntramyocellular lipidLC–MS/MSLipidomicsMass spectrometry imagingMuscle atrophy

Identifiers

PMID38725139
PMCPMC11294036

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.