ArticlePlant methods2024
A protoplast-based transient gene expression assay for the identification of heat and oxidative stress-regulatory genes in perennial ryegrass.
Article in Plant methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Genetic transformation of forage crops: comparative barriers, evidence, and emerging strategies.Plant cell reports · 2026Review
- LpCbDR1 regulates leaf senescence and drought tolerance by activating the chlorophyll b reductase gene and stress-related genes in perennial ryegrass.Horticulture research · 2026Article
- Characterization of oxidative status in maize protoplasts under temperature and saline-alkali stresses.BMC plant biology · 2026Article
- Phenotype microarray-based assessment of metabolic variability in plant protoplasts.Plant methods · 2025Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundWith the accumulating omics data, an efficient and time-saving transient assay to express target genes is desired. Mesophyll protoplasts, maintaining most stress-physiological responses and cellular activities as intact plants, offer an alternative transient assay to study target genes' effects on heat and oxidative stress responses.
resultsIn this study, a perennial ryegrass (Lolium perenne L.) mesophyll protoplast-based assay was established to effectively over- or down-regulate target genes. The relative expression levels of the target genes could be quantified using RT-qPCR, and the effects of heat and H
conclusionThis protoplast-based rapid stress regulatory gene identification assay was briefed as 'PRIDA' that will complement the stable genetic transformation studies to rapidly identify candidate stress-regulatory genes in perennial ryegrass and other grass species.
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