ArticleNature communications2024
A genetically encoded biosensor to monitor dynamic changes of c-di-GMP with high temporal resolution.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Genetically encoded tools for tracking metabolites in live cells.Biochemical Society transactions · 2026Review
- TheApplied and environmental microbiology · 2026Article
- Environmental regulation of virulence in Vibrio cholerae: integrating multi-omics, predictive models, and comparative insights across emerging Vibrio species.Gut pathogens · 2026Review
- Single-cell approach dissecting agr quorum sensing dynamics in Staphylococcus aureus.Nature communications · 2026Article
- The post-translational adaptor protein SadB modulates the pathogenicity ofJournal of bacteriology · 2026Article
- Network-level divergence in cyclic di-GMP signalling drives ecological versatility in Acinetobacter baumannii.NPJ biofilms and microbiomes · 2026Article
- Toolbox of FRET-based c-di-GMP biosensors and its FRET-To-Sort application for genome-wide mapping of c-di-GMP regulation.Nature communications · 2026Article
- groovDB in 2026: a community-editable database of small molecule biosensors.Nucleic acids research · 2026Article
- A Genetically Encoded Biosensor for Characterizing Transport and Metabolism of Glutarate.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Review
- A deoxynucleoside triphosphate triphosphohydrolase promotes cell cycle progression inJournal of bacteriology · 2025Article
- Pseudomonas syringae subpopulations cooperate by coordinating flagellar and type III secretion spatiotemporal dynamics to facilitate plant infection.Nature microbiology · 2025Article
- Review
- Editorial: Fusion proteins for the detection of pathogens or pathogen receptors.Frontiers in bioengineering and biotechnology · 2025Article
- Article
- Functional analysis of cyclic diguanylate-modulating proteins inbioRxiv : the preprint server for biology · 2024Article
- Toward DNA-Based Recording of Biological Processes.International journal of molecular sciences · 2024Review
- How c-di-GMP controls progression through the Streptomyces life cycle.Current opinion in microbiology · 2024Review
- A deterministic, c-di-GMP-dependent program ensures the generation of phenotypically similar, symmetric daughter cells during cytokinesis.Nature communications · 2024Article
- A genetically encoded biosensor to monitor dynamic changes of c-di-GMP with high temporal resolution.Nature communications · 2024Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Monitoring changes of signaling molecules and metabolites with high temporal resolution is key to understanding dynamic biological systems. Here, we use directed evolution to develop a genetically encoded ratiometric biosensor for c-di-GMP, a ubiquitous bacterial second messenger regulating important biological processes like motility, surface attachment, virulence and persistence. The resulting biosensor, cdGreen2, faithfully tracks c-di-GMP in single cells and with high temporal resolution over extended imaging times, making it possible to resolve regulatory networks driving bimodal developmental programs in different bacterial model organisms. We further adopt cdGreen2 as a simple tool for in vitro studies, facilitating high-throughput screens for compounds interfering with c-di-GMP signaling and biofilm formation. The sensitivity and versatility of cdGreen2 could help reveal c-di-GMP dynamics in a broad range of microorganisms with high temporal resolution. Its design principles could also serve as a blueprint for the development of similar, orthogonal biosensors for other signaling molecules, metabolites and antibiotics.
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Registered trials
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