Evidence map›Paper›PMID 38723628›Full record

ArticleCell2024

Deep mutational scanning of hepatitis B virus reveals a mechanism for cis-preferential reverse transcription.

Yingpu Yu, Maximilian A Kass, Mengyin Zhang, Noor Youssef, Catherine A Freije, Kelly P Brock, Lauren C Aguado, Leon L Seifert, Sanjana Venkittu, Xupeng Hong and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. The diverse functions of viral microproteins.bioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Hepatitis B Virus Nucleocapsid Assembly.Journal of molecular biology · 2025
    Review
  10. Article
  11. Review
  12. Article
  13. [Progress in clinical research related to viral hepatitis in 2024].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2025
    Article
  14. The role of ADAR1 in human pathophysiology.Frontiers in cell and developmental biology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yingpu YuLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
Maximilian A KassLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA; Department of Infectious Diseases, Molecular Virology, Heidelberg University, Medical Faculty Heidelberg, Heidelberg, Germany.
Mengyin ZhangLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
Noor YoussefDepartment of Systems Biology, Harvard Medical School, Boston, MA 02115, USA; Department of Organismic and Evolutionary Biology, Broad Institute of MIT and Harvard, Harvard University, Cambridge, MA 02138, USA.
Catherine A FreijeLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
Kelly P BrockDepartment of Systems Biology, Harvard Medical School, Boston, MA 02115, USA; Department of Organismic and Evolutionary Biology, Broad Institute of MIT and Harvard, Harvard University, Cambridge, MA 02138, USA.
Lauren C AguadoLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
Leon L SeifertLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA; Center for Clinical and Translational Science, The Rockefeller University, New York, NY 10065, USA.
Sanjana VenkittuLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
Xupeng HongLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
Amir ShlomaiLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA.
Ype P de JongLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA; Division of Gastroenterology and Hepatology, Weill Cornell Medical College, New York, NY 10065, USA.
Debora S MarksDepartment of Systems Biology, Harvard Medical School, Boston, MA 02115, USA; Department of Organismic and Evolutionary Biology, Broad Institute of MIT and Harvard, Harvard University, Cambridge, MA 02138, USA.
Charles M RiceLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA. Electronic address: ricec@rockefeller.edu.
William M SchneiderLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA. Electronic address: wschneider@rockefeller.edu.

Funding

Developing, Demonstrating, and Disseminating Innovative Programs to Achieve Translational SuccessUL1TR001866 · NCATS · ROCKEFELLER UNIVERSITY · PI COLLER, BARRY, KRUEGER, JAMES G · 2016 to 2025
$40.6M
Type I interferon-stimulated genes and the antiviral immune responseR01AI091707 · NIAID · ROCKEFELLER UNIVERSITY · PI RICE, CHARLES M · 2011 to 2020
$5.5M
Launching HBV with RNA to assess antiviral resistance and explore fundamental aspects of virus-host biologyR01AI150275 · NIAID · ROCKEFELLER UNIVERSITY · PI Charles M Rice · 2020 to 2026
$4.4M
In search of an HBV cure: novel model systems and targetsR01AI143295 · NIAID · ROCKEFELLER UNIVERSITY · PI RICE, CHARLES M · 2019 to 2023
$3.0M
NCATS NIH HHS UL1 TR001866NIAID NIH HHS R01 AI091707NIAID NIH HHS R01 AI143295NIAID NIH HHS R01 AI150275
6 · The paper itself

Abstract

Hepatitis B virus (HBV) is a small double-stranded DNA virus that chronically infects 296 million people. Over half of its compact genome encodes proteins in two overlapping reading frames, and during evolution, multiple selective pressures can act on shared nucleotides. This study combines an RNA-based HBV cell culture system with deep mutational scanning (DMS) to uncouple cis- and trans-acting sequence requirements in the HBV genome. The results support a leaky ribosome scanning model for polymerase translation, provide a fitness map of the HBV polymerase at single-nucleotide resolution, and identify conserved prolines adjacent to the HBV polymerase termination codon that stall ribosomes. Further experiments indicated that stalled ribosomes tether the nascent polymerase to its template RNA, ensuring cis-preferential RNA packaging and reverse transcription of the HBV genome.

Indexed as

Hepatitis B virusReverse TranscriptionCell LineGenome, ViralHumansMutationRibosomesRNA, ViralRNA, Viralcis preferencedeep mutational scanningdiprolinehepatitis B virusreverse transcriptionribosome stalling

Identifiers

PMID38723628
PMCPMC11127778

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.