Evidence map›Paper›PMID 38720329›Full record

ArticleBMC veterinary research2024

Development and evaluation of time-resolved rapid immunofluorescence test for detection of TSOL18 specific antibody in porcine cysticercosis infections.

Dejia Zhang, Rui Duan, Jing Liu, Mengqi Wang, Yi Yang, Yongjun Zhao, Mingyuan Liu, Shumin Sun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Dejia Zhang *College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China.
Rui Duan *College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China.
Jing Liu *Qingdao Special Servicemen Recuperation Center of PLA Navy, Qingdao, 266071, China.
Mengqi WangCollege of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China.
Yi YangCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, China.
Yongjun ZhaoCollege of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China.
Mingyuan LiuState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, 130062, China. liumy36@163.com.
Shumin SunCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, China. shums1975@163.com.

Funding

Inner Mongolia autonomous region science and technology plan project 2022YFDZ0049National Key Research and Development Program of China 2022YFE0114400the Basic scientific research operating expenses of colleges and universities directly under Inner Mongolia Autonomous Region project GXKY22042the National Nature Science Foundation of China 31460658the National Nature Science Foundation of China 32160842the Natural Science Foundation of Inner Mongolia Autonomous Region 2019LH03021the Natural Science Foundation of Inner Mongolia Autonomous Region 2021MS03037
6 · The paper itself

Abstract

backgroundPorcine cysticercosis, a serious zoonotic parasitic disease, is caused by the larvae of Taenia solium and has been acknowledged by the World Organization for Animal Health. The current detection methods of Cysticercus cellulosae cannot meet the needs of large-scale and rapid detection in the field. We hypothesized that the immunofluorescence chromatography test strip (ICS) for detecting Cysticercus cellulosae, according to optimization of a series of reaction systems was conducted, and sensitivity, specificity, and stability testing, and was finally compared with ELISA. This method utilizes Eu

resultsICS and autopsy have highly consistent diagnostic results (n = 133), as determined by Cohen's κ analysis (κ = 0.925). And the results showed that the proposed ICS are high sensitivity (0.9459) with specificity (0.9792). The ICS was unable to detect positive samples of other parasites. It can be stored for at least six months at 4℃.

conclusionsIn summary, we established a TRFM-ICS method with higher sensitivity and specificity than indirect ELISA. Results obtained from serum samples can be read within 10 min, indicating a rapid, user-friendly test suitable for large-scale field detection.

Indexed as

Antibodies, HelminthAntigens, HelminthCysticercosisEnzyme-Linked Immunosorbent AssayFluorescent Antibody TechniqueSensitivity and SpecificitySwine DiseasesAnimalsCysticercusSwineTaenia soliumAntibodies, HelminthAntigens, HelminthCysticercus cellulosaeImmunochromatographic stripImmunodiagnosisTime-resolved fluorescent microspheres

Identifiers

PMID38720329
PMCPMC11077887

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.