Evidence map›Paper›PMID 38717732›Full record

ArticleVeterinary research communications2024

Comparative characterisation of extracellular vesicles from canine and human plasma: a necessary step in biomarker discovery.

Stephanie Marie Bollard, J Howard, C Casalou, L Mooney, S Peters, C Sweeney, A Ajaykumar, K Triana, A McCann, P A Kelly and 1 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Stephanie Marie BollardUCD School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland. stephanie.bollard1@ucdconnect.ie.ORCID http://orcid.org/0000-0003-3220-6903
J HowardUCD School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
C CasalouUCD Charles Institute of Dermatology, University College Dublin, Belfield, Dublin 4, Ireland.
L MooneyUCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
S PetersUCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
C SweeneyUCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
A AjaykumarUCD Clinical Research Centre, University College Dublin, Belfield, Dublin 4, Ireland.
K TrianaUCD Clinical Research Centre, University College Dublin, Belfield, Dublin 4, Ireland.
A McCannUCD School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
P A KellyUCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
S M PotterUCD School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland.

Funding

Wellcome Trust
6 · The paper itself

Abstract

Extracellular Vesicles (EV) have become an interesting focus as novel biomarkers of disease and are increasingly reported upon in humans and other species. The Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV2018) guidelines were published to improve rigor and standardisation within the EV field and provide a framework for the reliable isolation and characterisation of EV populations. However, this rigor and standardisation has been challenging in the area of comparative medicine. Herein we present the successful isolation of EVs from human and canine plasma using Size Exclusion Chromatography and characterise these EVs according to best international practice. This study provides evidence for the reliable comparison of human and canine EVs isolated by this approach, and a baseline description of the EVs from healthy dogs to inform future biomarker studies. This work also demonstrates that the MISEV2018 guidelines can be successfully applied to EVs isolated from canine plasma.

Indexed as

BiomarkersChromatography, GelExtracellular VesiclesAnimalsDogsHumansBiomarkersCanineComparative medicineExtracellular vesiclesOneHealthSize exclusion chromatography

Identifiers

PMID38717732
PMCPMC11315736

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.