Evidence map›Paper›PMID 38716736›Full record

ArticleJournal of dental research2024

Neovascularization by DPSC-ECs in a Tube Model for Pulp Regeneration Study.

Y Zhang, J Liu, I J de Souza Araujo, L Bahammam, L L Munn, G T J Huang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dental research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Y ZhangDepartments of Bioscience Research and Endodontics, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA.
J LiuDepartments of Bioscience Research and Endodontics, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA.
I J de Souza AraujoDepartments of Bioscience Research and Endodontics, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA.
L BahammamDepartments of Bioscience Research and Endodontics, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA.ORCID 0000-0001-9075-361X
L L MunnRadiation Oncology, Massachusetts General Hospital Research Institute, Harvard Medical School, Charlestown, MA, USA.
G T J HuangDepartments of Bioscience Research and Endodontics, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA.

Funding

Pilot and Feasibility ProgramP30DK041301 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROZENGURT, JUAN ENRIQUE · 1990 to 2019
$18.0M
Kinetics of neo-vascularization during pulp regenerationR21DE029605 · NIDCR · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI HUANG, GEORGE T.J · 2021 to 2022
$418k
NIDCR NIH HHS R21 DE029605NIDDK NIH HHS P30 DK041301
6 · The paper itself

Abstract

The process of neovascularization during cell-based pulp regeneration is difficult to study. Here we developed a tube model that simulates root canal space and allows direct visualization of the vascularization process in vitro. Endothelial-like cells (ECs) derived from guiding human dental pulp stem cells (DPSCs) into expressing endothelial cell markers CD144, vWF, VEGFR1, and VEGFR2 were used. Human microvascular endothelial cells (hMVECs) were used as a positive control. DPSC-ECs formed tubules on Matrigel similar to hMVECs. Cells were mixed in fibrinogen/thrombin or mouse blood and seeded into wells of 96-well plates or injected into a tapered plastic tube (14 mm in length and 1 or 2 mm diameter of the apex opening) with the larger end sealed with MTA to simulate root canal space. Cells/gels in wells or tubes were incubated for various times in vitro and observed under the microscope for morphological changes. Samples were then fixed and processed for histological analysis to determine vessel formation. Vessel-like networks were observed in culture from 1 to 3 d after cell seeding. Cells/gels in 96-well plates were maintained up to 25 d. Histologically, both hMVECs and DPSC-ECs in 96-well plates or tubes showed intracellular vacuole formation. Some cells showed merged large vacuoles indicating the lumenization. Tubular structures were also observed resembling blood vessels. Cells appeared healthy throughout the tube except some samples (1 mm apical diameter) in the coronal third. Histological analysis also showed pulp-like soft tissue throughout the tube samples with vascular-like structures. hMVECs formed larger vascular lumen size than DPSC-ECs while the latter tended to have more lumen and tubular structure counts. We conclude that DPSC-ECs can form vascular structures and sustained in the 3-dimensional fibrin gel system in vitro. The tube model appears to be a proper and simple system simulating the root canal space for vascular formation and pulp regeneration studies.

Indexed as

Dental PulpDrug CombinationsEndothelial CellsNeovascularization, PhysiologicProteoglycansRegenerationStem CellsAluminum CompoundsAnimalsCalcium CompoundsCD146 AntigenCell Culture TechniquesCells, CulturedCollagenDental Pulp CavityFibrinogenAluminum CompoundsCalcium CompoundsCD146 AntigenCollagenDrug CombinationsFibrinogenLamininmatrigelMCAM protein, humanmineral trioxide aggregateOxidesProteoglycansRoot Canal Filling MaterialsSilicatesVascular Endothelial Growth Factor Receptor-2von Willebrand Factordental pulpendothelial cellsfibrinhydrogelsin vitroregeneration

Identifiers

PMID38716736
PMCPMC11122093

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.