ArticleEcology and evolution2024
Comparison of qPCR and metabarcoding for environmental DNA surveillance of a freshwater parasite.
Article in Ecology and evolution, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- A Noninvasive Fecal-DNA Detection Method for Sarcoptic Mange in the San Joaquin Kit Fox (Animals : an open access journal from MDPI · 2026Article
- Trends in Aquatic Environmental DNA Research in Alaska.Ecology and evolution · 2026Review
- Rapid Clearance of Tetracapsuloides bryosalmonae Spores by Freshwater Sponge Ephydatia muelleri: Potential Implications for Controlling Proliferative Kidney Disease in Salmonids.Journal of fish diseases · 2026Article
- High-throughput microfluidic real-time PCR as a promising tool in disease ecology.The Journal of animal ecology · 2025Article
- Fecal metabarcoding vs traditional invasive survey to access the diversity of anuran endoparasites and diet.Parasitology · 2025Article
- The Use of Environmental DNA as Preliminary Description of Invertebrate Diversity in Three Sicilian Lakes.Animals : an open access journal from MDPI · 2025Article
- Hazardous Materials from Threats to Safety: Molecularly Imprinted Polymers as Versatile Safeguarding Platforms.Polymers · 2024Review
- Comparison of qPCR and metabarcoding for environmental DNA surveillance of a freshwater parasite.Ecology and evolution · 2024Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analysis of environmental DNA (eDNA) has been successfully used across freshwater ecological parasitology to inform management of ecologically and economically important species. However, most studies have used species-specific quantitative polymerase chain reaction (qPCR) assays to detect target taxa. While generally effective, this approach limits the amount of community and management-supporting data that can be obtained from eDNA samples. If eDNA metabarcoding could be conducted with the same accuracy of a single species approach, researchers could simultaneously detect a target species while obtaining vast community data from eDNA samples. We sampled 38 freshwater sites on Fort McCoy, Wisconsin and compared qPCR to metabarcoding for eDNA detection of the ectoparasitic gill louse
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