ArticleAnalytical science advances2023
Assessment of ammonium fluoride as a mobile phase additive for sensitivity gains in electrospray ionization.
Article in Analytical science advances, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Advances in targeted liquid chromatography-tandem mass spectrometry methods for endocannabinoid and N-acylethanolamine quantification in biological matrices: A systematic review.Mass spectrometry reviewsPooled it
- Article
- Spatially resolved lipids in a mouse brain model of globoid cell leukodystrophy via IR-MALDESI MSI and parallel reaction monitoring MSI.Analytical and bioanalytical chemistry · 2026Article
- Exploring ammonium salt doping to enhance ion abundance for quantitative mass spectrometry imaging.Analytical and bioanalytical chemistry · 2025Article
- Signal enhancement of lipids and glycans using ammonium fluoride for IR-MALDESI mass spectrometry imaging.Analytical and bioanalytical chemistry · 2025Article
- Negative Paper Spray Ionization Mass Spectrometry for the Determination of Endocrine-Disrupting Chemicals with Application to Paraben Analysis in Cosmetics.Molecules (Basel, Switzerland) · 2025Article
- Effect of ammonium fluoride as a mobile phase additive on quantitativity of fatty acids in electrospray ionization-liquid chromatography-tandem mass spectrometry.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Mass Spectral Analysis of Sterols and Other Steroids in Different Ionization Modes: Sensitivity and Oxidation Artifacts.Journal of the American Society for Mass Spectrometry · 2025Article
- Determination of Hormonal Growth Promotants in Beef Using Liquid Chromatography-Tandem Mass Spectrometry.Drug testing and analysis · 2025Article
- Assessment of ammonium fluoride as a mobile phase additive for sensitivity gains in electrospray ionization.Analytical science advances · 2023Article
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Authors and funding
2 authors.
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Abstract
Ammonium fluoride has been shown to improve sensitivity when using electrospray ionization (ESI) coupled with mass spectrometry (MS). Recent internal investigation furthered that claim, through the observation of improved sensitivity when analyzing steroid molecules. This work focuses on extending those observations to other small molecules to understand the impact ammonium fluoride has on detection sensitivity with optimized instrument conditions. Using conventional liquid chromatography ESI-MS we investigated sensitivity differences between ammonium fluoride, formic acid, or ammonium hydroxide as mobile phase additives. Full source optimization was performed for nine compounds at three different organic concentrations (30%, 60%, or 90%) with formic acid, ammonium fluoride, and ammonium hydroxide adjustment. Optimization results were compiled to generate individual methods by compound, polarity, mobile phase, and organic concentration. Flow injection analysis was performed with fully optimized methods to compare compounds across different solvent systems under optimal conditions. Negative ESI data showed 2-22-fold sensitivity improvements for all compounds with ammonium fluoride. Positive ESI data showed > 1-11-fold improvement in sensitivity for four of seven compounds and no change for three of seven compounds with ammonium fluoride. Ammonium fluoride improved ESI
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