Evidence map›Paper›PMID 38715028›Full record

ArticleBMC veterinary research2024

Establishment of a real-time fluorescent quantitative PCR detection method and phylogenetic analysis of BoAHV-1.

Lihui Xu, Guiyang Ge, Dongli Li, Jianming Li, Qinglong Gong, Kun Shi, Fei Liu, Naichao Diao, Zhenzhen Cui, Yingyu Liu and 2 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Lihui Xu *College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Guiyang Ge *College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Dongli LiCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Jianming LiCollege of Chinese Medicine Materials, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Qinglong GongCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Kun ShiCollege of Chinese Medicine Materials, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Fei LiuCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Naichao DiaoCollege of Chinese Medicine Materials, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Zhenzhen CuiCollege of Chinese Medicine Materials, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Yingyu LiuCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Xue LengCollege of Chinese Medicine Materials, Jilin Agricultural University, Changchun, 130118, Jilin, China. xuel@jlau.edu.cn.
Rui DuCollege of Chinese Medicine Materials, Jilin Agricultural University, Changchun, 130118, Jilin, China. durui197101@sina.com.

Funding

This research was supported by the Science and Technology Development Project of Jilin Province 20220101332JC
6 · The paper itself

Abstract

backgroundInfectious bovine rhinotracheitis (IBR), caused by Bovine alphaherpesvirus-1 (BoAHV-1), is an acute, highly contagious disease primarily characterized by respiratory tract lesions in infected cattle. Due to its severe pathological damage and extensive transmission, it results in significant economic losses in the cattle industry. Accurate detection of BoAHV-1 is of paramount importance. In this study, we developed a real-time fluorescent quantitative PCR detection method for detecting BoAHV-1 infections. Utilizing this method, we tested clinical samples and successfully identified and isolated a strain of BoAHV-1.1 from positive samples. Subsequently, we conducted a genetic evolution analysis on the isolate strain's gC, TK, gG, gD, and gE genes.

resultsThe study developed a real-time quantitative PCR detection method using SYBR Green II, achieving a detection limit of 7.8 × 10

conclusionsThe established real-time fluorescent quantitative PCR detection method exhibits good repeatability, specificity, and sensitivity. Furthermore, genetic evolution analysis of the isolated BoAHV-1 JL-5 strain indicates that it belongs to the BoAHV-1.1 subtype. These findings provide a foundation and data for the detection, prevention, and control Infectious Bovine Rhinotracheitis.

Indexed as

AlphaherpesvirinaeInfectious Bovine RhinotracheitisReal-Time Polymerase Chain ReactionAnimalsCattlePhylogenySensitivity and SpecificitySpecimen HandlingBoAHV-1Phylogenic analysisRT‒qPCR

Identifiers

PMID38715028
PMCPMC11075196

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