Evidence map›Paper›PMID 38714547›Full record

ArticlePlanta2024

A functional study reveals CsNAC086 regulated the biosynthesis of flavonols in Camellia sinensis.

Sa-Sa Song, Wei-Xi Ran, Long-Han Gao, Yu-Chun Wang, Wu-Yun Lv, Yu Tao, Liang Chen, Chun-Fang Li

Abstract read
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Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sa-Sa Song *College of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou, 311300, China.
Wei-Xi Ran *College of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou, 311300, China.
Long-Han Gao *College of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou, 311300, China.
Yu-Chun WangCollege of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou, 311300, China.
Wu-Yun LvCollege of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou, 311300, China.
Yu TaoCollege of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou, 311300, China.
Liang ChenKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China. liangchen@tricaas.com.
Chun-Fang LiCollege of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou, 311300, China. lichunfang@zafu.edu.cn.ORCID http://orcid.org/0000-0002-6657-8548

Funding

Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, P. R. China TZDZW202203Natural Science Foundation of Zhejiang Province LY19C160003The Scientific Research Project of Zhejiang Education Department Y202353282
6 · The paper itself

Abstract

MAIN

conclusionCsNAC086 was found to promote the expression of CsFLS, thus promoting the accumulation of flavonols in Camellia sinensis. Flavonols, the main flavonoids in tea plants, play an important role in the taste and quality of tea. In this study, a NAC TF gene CsNAC086 was isolated from tea plants and confirmed its regulatory role in the expression of flavonol synthase which is a key gene involved in the biosynthesis of flavonols in tea plant. Yeast transcription-activity assays showed that CsNAC086 has self-activation activity. The transcriptional activator domain of CsNAC086 is located in the non-conserved C-terminal region (positions 171-550), while the conserved NAC domain (positions 1-170) does not have self-activation activity. Silencing the CsNAC086 gene using antisense oligonucleotides significantly decreased the expression of CsFLS. As a result, the concentration of flavonols decreased significantly. In overexpressing CsNAC086 tobacco leaves, the expression of NtFLS was significantly increased. Compared with wild-type tobacco, the flavonols concentration increased. Yeast one-hybrid assays showed CsNAC086 did not directly regulate the gene expression of CsFLS. These findings indicate that CsNAC086 plays a role in regulating flavonols biosynthesis in tea plants, which has important implications for selecting and breeding of high-flavonols-concentration containing tea-plant cultivars.

Indexed as

Camellia sinensisFlavonolsGene Expression Regulation, PlantNicotianaPlant ProteinsOxidoreductasesPlant LeavesPlants, Genetically ModifiedTranscription FactorsFlavonolsflavonol synthaseOxidoreductasesPlant ProteinsTranscription FactorsAntisense oligonucleotidesCamellia sinensisFlavonolsFlavonol synthaseNAC transcription factor

Identifiers

PMID38714547

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.