ArticlePlant physiology2024
A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901.
Article in Plant physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Targeted genome editing of the non-model cyanobacterium Cyanothece PCC 7425 via CRISPR/Cas12a.Applied microbiology and biotechnology · 2026Article
- Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression.Current issues in molecular biology · 2026Review
- Systematic Evaluation of Signal Peptide-Driven Protein Secretion in the Fast-Growing CyanobacteriumBiomolecules · 2026Article
- Toward Sustainability: Intensification of Light-Driven Whole Cell Biocatalysis.Chembiochem : a European journal of chemical biology · 2026Review
- Living Photoanodes for Solar-Driven Water Oxidation.Chemical reviews · 2026Review
- Green genes from blue greens: challenges and solutions to unlocking the potential of cyanobacteria in drug discovery.Natural product reports · 2026Review
- CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.BMC biotechnology · 2026Article
- A Bioprocess Engineering Approach to Boost Selection of Fully Segregated Transformants in Cyanobacteria.Biotechnology and bioengineering · 2025Article
- Establishing a Malonyl-CoA Biosensor for the Two Model CyanobacteriaACS synthetic biology · 2025Article
- Engineering of the fast-growing cyanobacterium Synechococcus sp. PCC 11901 to synthesize astaxanthin.Biotechnology for biofuels and bioproducts · 2025Article
- Biotechnological Applications of Cyanobacteria: Synechocystis and Synechococcus Strains.Advances in biochemical engineering/biotechnology · 2025Review
- Review of recent advances in improvement strategies for biofuels production from cyanobacteria.Heliyon · 2024Article
- New molecular tools for the next generation of solar microbial cell factories.Plant physiology · 2024Article
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7 authors.
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Abstract
Synechococcus sp. PCC 11901 (PCC 11901) is a fast-growing marine cyanobacterial strain that has a capacity for sustained biomass accumulation to very high cell densities, comparable to that achieved by commercially relevant heterotrophic organisms. However, genetic tools to engineer PCC 11901 for biotechnology applications are limited. Here we describe a suite of tools based on the CyanoGate MoClo system to unlock the engineering potential of PCC 11901. First, we characterized neutral sites suitable for stable genomic integration that do not affect growth even at high cell densities. Second, we tested a suite of constitutive promoters, terminators, and inducible promoters including a 2,4-diacetylphloroglucinol (DAPG)-inducible PhlF repressor system, which has not previously been demonstrated in cyanobacteria and showed tight regulation and a 228-fold dynamic range of induction. Lastly, we developed a DAPG-inducible dCas9-based CRISPR interference (CRISPRi) system and a modular method to generate markerless mutants using CRISPR-Cas12a. Based on our findings, PCC 11901 is highly responsive to CRISPRi-based repression and showed high efficiencies for single insertion (31% to 81%) and multiplex double insertion (25%) genome editing with Cas12a. We envision that these tools will lay the foundations for the adoption of PCC 11901 as a robust model strain for engineering biology and green biotechnology.
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