Evidence map›Paper›PMID 38713004›Full record

ArticleBiology open2024

Na,K-ATPase activity promotes macropinocytosis in colon cancer via Wnt signaling.

Nydia Tejeda-Muñoz, Yagmur Azbazdar, Eric A Sosa, Julia Monka, Pu-Sheng Wei, Grace Binder, Kuo-Ching Mei, Yerbol Z Kurmangaliyev, Edward M De Robertis

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nydia Tejeda-MuñozDepartment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles 90095-1662, USA.ORCID 0000-0001-8314-412X
Yagmur AzbazdarDepartment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles 90095-1662, USA.
Eric A SosaDepartment of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.ORCID 0000-0001-5965-3477
Julia MonkaDepartment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles 90095-1662, USA.
Pu-Sheng WeiDepartment of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, State University of New York at Binghamton, Binghamton, Johnson City, NY 13790, USA.
Grace BinderDepartment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles 90095-1662, USA.
Kuo-Ching MeiDepartment of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, State University of New York at Binghamton, Binghamton, Johnson City, NY 13790, USA.ORCID 0000-0001-6045-2154
Yerbol Z KurmangaliyevDepartment of Biology, Brandeis University, Waltham, MA 02453, USA.
Edward M De RobertisDepartment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles 90095-1662, USA.

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Damoiseaux · 1985 to 2026
$134.5M
Medical Scientist Training ProgramT32GM149364 · NIGMS · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI Myles H. Akabas · 2023 to 2026
$7.5M
Defining the gene regulatory roles of non-coding variants in the pathogenesis of autismF31MH131380 · NIMH · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI SOSA, ERIC ALEXANDER · 2022 to 2025
$213k
NCI NIH HHS P30 CA016042NIGMS NIH HHS T32 GM149364NIH HHS P20CA016042NIMH NIH HHS F31 MH131380Oklahoma UniversityOUHSC: The University of Oklahoma Health Sciences CenterStephenson Cancer CenterSUNY Binghamton faculty startup fund 910252-69SUNY Binghamton TAE Seed Grant RF1182424University of California, Los Angeles Jonsson Comprehensive Cancer Center, the Norman Sprague Endowment for Molecular Oncology UCLA BD-55
6 · The paper itself

Abstract

Recent research has shown that membrane trafficking plays an important role in canonical Wnt signaling through sequestration of the β-catenin destruction complex inside multivesicular bodies (MVBs) and lysosomes. In this study, we introduce Ouabain, an inhibitor of the Na,K-ATPase pump that establishes electric potentials across membranes, as a potent inhibitor of Wnt signaling. We find that Na,K-ATPase levels are elevated in advanced colon carcinoma, that this enzyme is elevated in cancer cells with constitutively activated Wnt pathway and is activated by GSK3 inhibitors that increase macropinocytosis. Ouabain blocks macropinocytosis, which is an essential step in Wnt signaling, probably explaining the strong effects of Ouabain on this pathway. In Xenopus embryos, brief Ouabain treatment at the 32-cell stage, critical for the earliest Wnt signal in development-inhibited brains, could be reversed by treatment with Lithium chloride, a Wnt mimic. Inhibiting membrane trafficking may provide a way of targeting Wnt-driven cancers.

Indexed as

Colonic NeoplasmsPinocytosisSodium-Potassium-Exchanging ATPaseWnt Signaling PathwayAnimalsCell Line, TumorHumansOuabainXenopusOuabainSodium-Potassium-Exchanging ATPaseColorectal carcinomaK-ATPaseMacropinocytosisMultivesicular bodiesNaOuabainWnt signalingXenopus laevis

Identifiers

PMID38713004
PMCPMC11139033

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.