Evidence map›Paper›PMID 38712170›Full record

ArticlebioRxiv : the preprint server for biology2024

Biochemical and structural insights into a 5' to 3' RNA ligase reveal a potential role in tRNA ligation.

Yingjie Hu, Victor A Lopez, Hengyi Xu, James P Pfister, Bing Song, Kelly A Servage, Masahiro Sakurai, Benjamin T Jones, Joshua T Mendell, Tao Wang and 5 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Yingjie HuDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.ORCID 0000-0003-3103-525X
Victor A LopezDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Hengyi XuDepartments of Molecular Biosciences and Oncology, University of Texas at Austin, Austin, Texas 78712, USA.
James P PfisterDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Bing SongQuantitative Biomedical Research Center, Peter O'Donnell Jr. School of Public Health, UT Southwestern Medical Center, Dallas, Texas 75390, USA.
Kelly A ServageDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Masahiro SakuraiDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Benjamin T JonesDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Joshua T MendellDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.ORCID 0000-0001-8479-2284
Tao WangQuantitative Biomedical Research Center, Peter O'Donnell Jr. School of Public Health, UT Southwestern Medical Center, Dallas, Texas 75390, USA.
Jun WuDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Alan M LambowitzDepartments of Molecular Biosciences and Oncology, University of Texas at Austin, Austin, Texas 78712, USA.
Diana R TomchickDepartment of Biophysics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Krzysztof PawłowskiDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Vincent S TagliabracciDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.

Funding

Breathing life into dead enzymesDP2GM137419 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI TAGLIABRACCI, VINCENT SCOTT · 2019 to 2020
$2.6M
The role of long noncoding RNA CRNDE in normal physiology and cancerR01CA282036 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI Joshua T Mendell · 2023 to 2026
$1.9M
Dissect the mechanisms underlying interspecies pluripotent stem cell competitionR01HD103627 · NICHD · UT SOUTHWESTERN MEDICAL CENTER · PI Michael Buszczak, Ondine B Cleaver · 2022 to 2026
$1.8M
NCI NIH HHS R01 CA282036NICHD NIH HHS R01 HD103627NIGMS NIH HHS DP2 GM137419
6 · The paper itself

Abstract

ATP-grasp superfamily enzymes contain a hand-like ATP-binding fold and catalyze a variety of reactions using a similar catalytic mechanism. More than 30 protein families are categorized in this superfamily, and they are involved in a plethora of cellular processes and human diseases. Here we identify C12orf29 as an atypical ATP-grasp enzyme that ligates RNA. Human C12orf29 and its homologs auto-adenylate on an active site Lys residue as part of a reaction intermediate that specifically ligates RNA halves containing a 5'-phosphate and a 3'-hydroxyl. C12orf29 binds tRNA in cells and can ligate tRNA within the anticodon loop

Indexed as

C12orf29Research reportsRNA ligasetRNA

Identifiers

PMID38712170
PMCPMC11071452

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.